Browsing 03 SEAFDEC/AQD External Publications by Author "Palma, Peter"
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Aeromonas load and species composition in tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines
Aeromonas load and species composition in the rearing water, sediment, gills and intestines of healthy tilapia Oreochromis niloticus collected every 2 weeks from Days 30 to 120 after stocking in six earthen ponds in the ... -
Aquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptide
Chieu, Hoang Dinh; Turner, Luke; Smith, Meaghan K.; Wang, Tianfang; Nocillado, Josephine; Palma, Peter ; Suwansa-ard, Saowaros ; Elizur, Abigail; Cummins, Scott F. (Frontiers Media, 2019-02-19)Wild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea ... -
Developing technologies for giant grouper (Epinephelus lanceolatus) aquaculture in Vietnam, the Philippines and Australia
Nocillado, Josephine ; Elizur, Abigail ; Palma, Peter ; Dennis, Lachlan; Ninh, Nguyen Huu; de Jesus-Ayson, Evelyn Grace T.; In, Vu Van; Thai, Truong Quoc; Anderson, Kelli ; Luu, Thi Ha Giang; Knibb, Wayne ; Knuckey, Richard; Canépa, Maximiliano; Bright, David (Australian Centre for International Agricultural Research, 2021-06-29)This project established a sustainable aquaculture industry for giant grouper in Vietnam and the Philippines through the development of captive breeding and larval-rearing techniques. Captive breeding innovations and ... -
Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
Peter Dennis, Lachlan; Nocillado, Josephine; Palma, Peter ; Amagai, Takafumi; Soyano, Kiyoshi; Elizur, Abigail (Elsevier, 2020-07-13)A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits ... -
Development of specific enzyme-linked immunosorbent assay for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone using recombinant gonadotropins
Nocillado, Josephine; Palma, Peter ; Fielder, Stewart; Zanardini, Maya; Dennis, Lachlan P.; Elizur, Abigail (Elsevier, 2019)We developed a specific competitive enzyme-linked immunosorbent assay (ELISA) for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone (FSH). We previously produced a full-length single chain recombinant ... -
Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration
Palma, Peter ; Nocillado, Josephine; Superio, Joshua; Ayson, Evelyn Grace de Jesus; Ayson, Felix; Bar, Ido; Elizur, Abigail (Oxford University Press, 2019-03)The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in ... -
Induction of gonadal development in protogynous grouper with orally delivered FSH DNA
Palma, Peter ; Nocillado, Josephine; Superio, Joshua; de Jesus-Ayson, Evelyn Grace; Ayson, Felix; Takemura, Akihiro; Lu, Ming Wei; Elizur, Abigail (Springer, 2019-08-01)The availability of sexually mature fish often dictates the success of its captive breeding. In this study, we induced reproductive development in juvenile protogynous tiger grouper through oral administration of a plasmid ... -
Insights on the seasonal variations of reproductive features in the eastern Atlantic bluefin tuna
Carnevali, Oliana; Maradonna, Francesca; Sagrati, Andrea; Candelma, Michela; Lombardo, Francesco; Pignalosa, Paolo; Bonfanti, Erica; Nocillado, Josephine; Palma, Peter ; Gioacchini, Giorgia; Elizur, Abigail (Elsevier, 2019)The Atlantic Bluefin Tuna (ABFT, Thunnus thynnus) is one of the most intensely exploited fisheries resources in the world. In spite of the years of studies on ABFT, basic aspects of its reproductive biology remain uncertain. ... -
Intracellular production of recombinant GnRH1 in yeast, Pichia pastoris, and its potential as oral treatment to advance gonadal development in juvenile orange-spotted grouper, Epinephelus coioides
Nocillado, Josephine ; Palma, Peter ; Wang, Tianfang ; de Jesus-Ayson, Evelyn Grace T.; Levavi-Sivan, Berta ; Elizur, Abigail (Elsevier, 2022-05)Late maturation and large size at maturity are significant challenges when administering hormonal treatments in captive broodstock. Here, we report the potential of yeast, Pichia pastoris, as a vehicle to orally deliver a ... -
Motile aeromonads recovered from tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines: Assessment of antibiotic susceptibility and multidrug resistance to selected antibiotics
Pakingking, Rolando V., Jr. ; Espanola, Jasca Gayle ; Palma, Peter ; Usero, Roselyn (AquacultureHub Inc., 2022-07)A total of 177 motile Aeromonas spp., comprised of A. hydrophila (n=162) and A. sobria (n=15), isolated from the water, sediment, gills and intestines of tilapia collected from the grow-out culture earthen ponds in the ... -
Quantitative and qualitative analyses of the bacterial microbiota of tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines
The quantity and composition of the bacterial microbiota in the rearing water, sediment, gills and intestines of tilapia Oreochromis niloticus collected every 2 weeks from Day 30 to Day 120 after stocking for grow-out ... -
Reproductive development of the threatened giant grouper Epinephelus lanceolatus
Palma, Peter ; Takemura, Akihiro; Libunao, Gardel Xyza; Superio, Joshua; de Jesus-Ayson, Evelyn Grace; Ayson, Felix; Nocillado, Josephine; Dennis, Lachlan; Chan, Josielou; Thai, Truong Quoc; Ninh, Nguyen Huu; Elizur, Abigail (Elsevier, 2019-05-02)The giant grouper is presumed to follow the reproductive pattern of most Epinephelus species, characterized by protogynous hermaphroditism wherein male maturation is attained through sex reversal of a functional female. ... -
Seasonal population dynamics of Lepeophtheirus spinifer and Neobenedenia sp. coinfecting snubnose pompano (Trachinotus blochii) breeders in marine cages in the Philippines
Palma, Peter ; Beluso, Lyra Angelica; de Jesus-Ayson, Evelyn Grace; Cruz-Lacierda, Erlinda R. (Elsevier, 2022)Studies on the biology and ecology of sea lice are lacking in tropical regions such as in Southeast Asia where finfish cage farming has grown dramatically in the past decades. This study investigated the seasonal population ... -
Studies towards advancing reproductive development in giant grouper (Epinephelus lanceolatus Bloch) using recombinant hormone manipulation
Groupers are an economically significant commodity owing to the major portion they occupy in the live reef fish trade (LRFT). The giant grouper E. lanceolatus is one of the most sought-after grouper species in the LRFT, ... -
The use of potassium permanganate against trichodiniasis on milkfish (Chanos chanos) fingerlings
Palma, Peter ; Cruz-Lacierda, Erlinda R.; Corre, Valeriano L., Jr. (European Association of Fish Pathologists, 2015)Trichodiniasis was noted in an intensive milkfish (Chanos chanos) nursery pond in Dumangas, Iloilo, Philippines. It was predominantly caused by a small trichodinid species (body diameter=23-29 µm) with well-developed ... -
Vibrio load and percentage composition of sucrose‐fermenting vibrios in Nile tilapia (Oreochromis niloticus) cultured in brackish water earthen ponds and biocontrol potential of recycled tilapia‐conditioned greenwater against population growth of V. parahaemolyticus and non‐sucrose‐fermenting vibrios in the grow‐out culture of white leg shrimp (Litopenaeus vannamei)
Pakingking, Rolando V., Jr. ; Palma, Peter ; de Jesus-Ayson, Evelyn Grace T.; Usero, Roselyn (Wiley, 2022-10-20)Vibrio are known opportunistic pathogens of pond-cultured fish and shrimp. This study determined the presumptive Vibrio count (PVC) and percentage composition of “beneficial” sucrose-fermenting vibrios (SFVs) ...