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dc.contributor.authorJing, Ng Hong
dc.contributor.authorTaha, Aishah Mohd
dc.contributor.authorPakingking Jr., Rolando V.
dc.contributor.authorWahab, Roswanira A. B.
dc.contributor.authorHuyop, Fahrul
dc.identifier.citationJing NH, Taha AM, Pakingking RV Jr., Wahab RAB, Huyop F. 2008. Dehalogenase from Methylobacterium sp. HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon). African Journal of Microbiology Research 2(2):32-36.en
dc.description.abstractHeavy industrial activities and agricultural processes require consumption of many halogenated compounds, and release them continuously as pollutants into the environment. These xenobiotics show high toxicity and persistence and cause many problems to the society, soils and ground water. Microbial dehalogenases are involved in the biodegradation of many important chlorinated compounds. A bacterial strain identified as Methylobacterium sp. HJ1 is able to degrade the herbicide 2,2-dichloropropionic acid by removal of the halogen and subsequent metabolism of the product for energy. D,L-2-chloropropionate also supported good growth of the organism but 3-chloropropionate, monochloroacetate and dichloroacetate were not utilized. Cell-free extracts of the 2,2-dichloropropionate-grown bacteria converted 2,2-dichloropropionate into pyruvate with the release of two chloride ions for each molecule of pyruvate formed. This indicates the presence of dehalogenase activity in the cell-free extracts. Only 2,2-dichloropropionate and D,L-2-chloropropionate were inducers and substrates for the dehalogenase. Monochloroacetate and dichloroacetate did not serve as an inducer, whereas 3-chloropropionate was a non-substrate inducer.en
dc.publisherAcademic Journalsen
dc.relation.ispartofAfrican Journal of Microbiology Research 2:32-36en
dc.subject2,2-dichloropropionic aciden
dc.subjectMethylobacterium sp.en
dc.titleDehalogenase from Methylobacterium sp. HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)en
dc.citation.journalTitleAfrican Journal of Microbiology Researchen

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  • AQD [1129]
    These papers were contributed by AQD staff to various national and international journals

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