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dc.contributor.authorTan-Fermin, Josefa D.
dc.contributor.authorMarte, Clarissa L.
dc.contributor.authorUeda, Hiroshi
dc.contributor.authorAdachi, Shinji
dc.contributor.authorYamauchi, Kohei
dc.date.accessioned2012-07-23T06:50:37Z
dc.date.available2012-07-23T06:50:37Z
dc.date.issued1999
dc.identifier.citationTan-Fermin, J. D., Marte, C. L., Ueda, H., Adachi, S., & Yamauchi, K. (1999). Effect of season on oocyte development and serum steroid hormones in LHRHa and pimozide-injected catfish Clarias macrocephalus (Günther). Fisheries Science, 65(6), 865–870.en
dc.identifier.issn0919-9268
dc.identifier.urihttp://hdl.handle.net/10862/1002
dc.description.abstractOocyte and blood samples were taken from gravid female catfish Clarias macrocephalus at 4-h intervals to monitor the stage of oocyte development and serum steroid hormone profiles after injection of luteinizing hormone-releasing hormone analogue (LHRHa) and pimozide (PIM) during the off-season (February) and the peak of the natural breeding period (August). Results showed that the onset of final oocyte maturation (12h) and ovulation (16h), and levels of serum estradiol-17β (E<sub>2</sub>) did not vary with season in LHRHa+PIM-injected fish. In February, ovulated eggs were stripped from three and two hormone-treated fish at 16h and 20h post-injection, respectively. In August, ovulation was observed in all hormone-treated females (n=5) at 16h post-injection but stripping of the eggs was possible only 4h thereafter. Serum E<sub>2</sub> levels were significantly different only with varying time post-injection; a marked increase occurred at 12h, but the elevation was higher in fish induced to ovulate during the peak (16.8ng/ml) than off-season (7.7ng/ml). Hormone-treated fish showed higher serum testosterone (T) levels during the peak season (17-23ng/ml) than those injected during the off-season (10-20ng/ml) at 4-12h post-injection. Serum 17α, 20β-dihydroxy-4-pregnene-3-one (DHP) levels of hormone-treated fish during the off-season were only about half the level (0.29 and 0.52 ng/ml) of those treated with the same hormones during the peak season (0.54 and 0.9ng/ml) at 8 and 12h postinjection, respectively. Development of oocytes and serum steroid hormone profiles after LHRHa+PIM-induced ovulation provide basic understanding of the processes that mediate final oocyte maturation and ovulation in captive C. macrocephalus.en
dc.language.isoenen
dc.publisherJapanese Society of Fisheries Scienceen
dc.subjectClarias macrocephalus
dc.subjectฤดูกาล
dc.subjectฮอร์โมนสเตอรอยด์en
dc.subjectCatfishen
dc.titleEffect of season on oocyte development and serum steroid hormones in LHRHa and pimozide-injected catfish Clarias macrocephalus (Günther)en
dc.typeArticleen
dc.citation.volume65
dc.citation.issue6
dc.citation.spage865
dc.citation.epage870
dc.citation.journalTitleFisheries Scienceen
dc.subject.asfaการเพาะเลี้ยงปลาen
dc.subject.asfafreshwater aquacultureen
dc.subject.asfaInduced breedingen
dc.subject.asfaLuteinizing hormone-releasing hormoneen
dc.subject.asfaเซลล์ไข่en
dc.subject.asfaกระบวนการสร้างเซลล์ไข่en
dc.subject.asfaการตกไข่en
dc.subject.asfaฮอร์โมนเพศen
dc.subject.asfaspawning seasonsen
dc.subject.asfaสเตอรอยด์en


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  • AQD [1133]
    These papers were contributed by AQD staff to various national and international journals

แสดงระเบียนรายการที่เรียบง่าย