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dc.contributor.authorHara, Shiro
dc.contributor.authorCanto Jr., Jose T.
dc.contributor.authorAlmendras, Jesus M. E.
dc.date.accessioned2012-08-24T09:15:56Z
dc.date.available2012-08-24T09:15:56Z
dc.date.issued1982
dc.identifier.citationHara, S., Canto Jr., J.T., & Almendras, J.M.E. (1982). A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28(3-4), 339-346. doi:10.1016/0044-8486(82)90076-Xen
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/1041
dc.description.abstractVarious extenders, containing potassium chloride, sodium chloride, glucose, sodium citrate, Ringer's solution, cow serum and milkfish serum were used to preserve milkfish (Chanos chanos) sperm at near-zero temperatures (0-4°C) and in liquid nitrogen (?196°C). Milkfish serum was a superior extender in both cases. After 5 days, comparatively good motility (> 30%) and fertilizing capacity (6.7-18.9%) were observed in the near-zero liquid samples, while in other extenders, sperm ceased to show motility after 2 days. The fertilization success of 4-5 days cryopreserved sperm averaged 67.5% (n = 2) with milkfish serum, 60.5% (n = 2) with 400 mM glucose, 58.0% (n = 2) with 150 mM sodium chloride, 41.2% (n = 1) with Ringer's solution and 31.9% (n = 2) with cow serum.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectChanos chanosen
dc.subjectSpermen
dc.subjectPreservativesen
dc.subjectFreezing storageen
dc.subjectAquaculture techniquesen
dc.subjectMilkfishen
dc.subjectFertilizationen
dc.titleA comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservationen
dc.typeArticleen
dc.citation.volume28
dc.citation.issue3-4
dc.citation.spage339
dc.citation.epage346
dc.citation.journalTitleAquacultureen
dc.identifier.doi10.1016/0044-8486(82)90076-X


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  • AQD [1128]
    These papers were contributed by AQD staff to various national and international journals

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