Ascorbic acid sulfate sulfohydrolase (C2 sulfatase): the modulator of cellular levels of L-ascorbic acid in rainbow trout.
MetadataShow full item record
The enzyme L-ascorbic acid 2-sulfate sulfohydrolase (C2 sulfatase) was purified from rainbow trout liver. The enzyme catalyzes the hydrolysis of L-ascorbic acid 2-sulfate and has a pH optimum at 6.0. It has a molecular weight of about 117,500 at pH 5.0 and is inhibited by a number of sulfhydryl blocking agents including L-ascorbic acid. C2 sulfatase activity was observed in most metabolic organs of rainbow trout. These findings suggest that the physiologic role of the enzyme is to maintain adequate cellular concentrations of L-ascorbic acid in the fish. The activity of the enzyme is controlled by L-ascorbic acid through feedback inhibition. Comparison of kinetic constants and inhibition patterns suggests that C2 sulfatase is structurally identical to human arylsulfatase A. However, unlike C2 sulfatase, human arylsulfatase A may not be involved in ascorbate metabolism. Its physiologic substrate is reported to be cerebroside-3-sulfate, not L-ascorbic acid 2-sulfate. A scheme is proposed to account for the functional divergence of these two structurally identical enzymes.
Suggested CitationBenitez, L. V., & Halver, J. E. (1982). Ascorbic acid sulfate sulfohydrolase (C2 sulfatase): the modulator of cellular levels of L-ascorbic acid in rainbow trout.
- AQD Journal Articles 
Showing items related by title, author, creator and subject.
Analysis of additives: Determination of sorbic acid, benzoic acid and dehydroacetic acid by steam distillation and UV spectrophotometric method Yamagata, Makoto (Marine Fisheries Research Department, Southeast Asian Fisheries Development Center, 1992)
Benitez, Lita V. (Asian Fisheries Society, 1989)The amino acid profile is an important parameter in the evaluation of protein quality and in requirement studies. Amino acid profiles are usually determined in 2 steps: hydrolysis of the protein to constituent amino acids ...
Evaluation of free essential amino acid in muscle of Nile tilapia (Oreochromis niloticus), as a basis of amino acid requirement for growth. Santiago, C.B.; Lovell, R.T. (Asian Fisheries Society, 1994)The free essential amino acid levels in muscle of young Nile tilapia (Oreochromis niloticus) after feeding graded amounts of a corresponding test amino acid were measured in 9 separate experiments to determine if free amino ...