The essential fatty acid requirement of milkfish (Chanos chanos Forsskal)
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The essential fatty acid (EFA) requirement of milkfish was examined by a 12-week feeding trial using defined, purified diets at water temperature of 28–29°C and salinity of 32‰. The test diets contained varying levels of 18:0 (triglyceride form, TG), 18:3(n−3), 18:2(n−6) and (n−3) highly unsaturated fatty acids (n−3 HUFA). Milkfish juveniles were starved for 7 days and were than fed lipid-free diet for 30 days before the initiation of feeding trials. Low growth and feed efficiency together with high mortalities were observed in fish fed the lipid-free diet as well as in the EFA-deficient diet. Supplementation of 2% 18:2(n−6) to the tristearin based diet did not improve growth rate of milkfish as effectively as feeding with (n−3) fatty acids. The highest weight gain was obtained in milkfish fed a combination of 5% 18:0 + 1.0% 18:3(n−3) + 0.5% 20:5(n−3) + 0.5% 22:6(n−3) although the supplementation of 2% 18:3(n−3) alone or combination of 0.5% 20:5(n−3) + 0.5% 22:6(n−3) to the tristearin based diets were also effective for improvement of growth. Thus, (n−3) fatty acids, such as 18:3(n−3) and (n−3)HUFA were nutritionally more important than 18:2(n−6) for milkfish. The fatty acid composition of the polar lipids from whole body of milkfish juveniles fed the various test diets were influenced by the composition of the dietary fatty acids.
การอ้างอิงBorlongan, I. G. (1992). The essential fatty acid requirement of milkfish (Chanos chanos Forsskal).
- AQD 
Benitez, Lita V. (Philippine Council for Aquatic and Marine Research and Development, 1989)This paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and ...
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) Requintina, Pura D.; Engle, L. M.; Benitez, Lita V. (University of the Philippines at Los Baños, 1981)Polyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were ...
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