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Cloning of IGF-I, IGF-II and IGF-IR cDNAs in mullet (Mugil cephalus) and grouper (Epinephelus coioides): Molecular markers for egg quality in marine fish

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dc.contributor.authorGonzaga, Josette
dc.contributor.authorAnderson, A.
dc.contributor.authorRichardson, N.
dc.contributor.authorNocillado, J.
dc.contributor.authorElizur, A.
dc.date.accessioned2014-05-28T06:27:26Z
dc.date.available2014-05-28T06:27:26Z
dc.date.issued2010
dc.identifier.citationGonzaga, J., Anderson, A., Richardson, N., Nocillado, J., & Elizur, A. (2010). Cloning of IGF-I, IGF-II and IGF-IR cDNAs in mullet (Mugil cephalus) and grouper (Epinephelus coioides): Molecular markers for egg quality in marine fish. Asian Journal of Biological Sciences, 3(2), 55-67.en
dc.identifier.issn1996-3351
dc.identifier.urihttp://hdl.handle.net/10862/2102
dc.description.abstractThe putative cDNA sequence of insulin like growth factor (IGF)-I, IGF-II and IGF-IR (receptor) was determined from the tissues of mullet, Mugil cephalus and grouper Epinephelus coioides. The deduced partial coding sequences were deposited at Gene Bank (Accession No. AY427954, AY427955, AY772254, AY776158, AY776159, AY772255). These basic data were used to determine messenger ribonucleic acid (mRNA) levels during embryogenesis in the two species. Results showed that IGF-II has the highest expression ratio and makes it a potential marker for egg quality in the two cultured species.en
dc.description.sponsorshipThe authors would like to acknowledge the assistance of Hazra Thaggard and Dr. Mike Rimmer in the sample collection. This study was part of the John Allwright Fellowship of the Australian Centre for International Agricultural Research with the project collaboration of SEAFDEC/AQD granted to JG (2002-2004). A. Elizur, A. Anderson and N. Richardson provided concept for the work, J. Nocillado helped design primers for amplification of the target genes and assisted in the sample and tissue collection and J. Gonzaga performed gene cloning, mRNA expression assay and statistical analysis.en
dc.language.isoenen
dc.publisherKnowledgia Scientificen
dc.relation.urihttp://www.scialert.net/qredirect.php?doi=ajbs.2010.55.67&linkid=pdf
dc.subjectgrowth factorsen
dc.subjectegg shellen
dc.subjectmulletsen
dc.subjectMugil cephalusen
dc.subjectgroupersen
dc.subjectEpinephelus coioidesen
dc.titleCloning of IGF-I, IGF-II and IGF-IR cDNAs in mullet (Mugil cephalus) and grouper (Epinephelus coioides): Molecular markers for egg quality in marine fishen
dc.typeArticleen
dc.citation.volume3
dc.citation.issue2
dc.citation.spage55
dc.citation.epage67
dc.citation.journalTitleAsian Journal of Biological Sciencesen
dc.subject.asfagrowthen
dc.subject.asfagenesen
dc.subject.asfaqualityen
dc.subject.asfahybridizationen
dc.subject.asfaaluminiumen
dc.subject.asfagenetic markersen
dc.subject.asfastatistical analysisen
dc.subject.asfaRNAen
dc.identifier.doi10.3923/ajbs.2010.55.67


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    These papers were contributed by AQD staff to various national and international journals

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