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dc.contributor.authorAmar, Edgar C.
dc.contributor.authorFaisan Jr., Joseph P.
dc.contributor.authorApines-Amar, Mary Jane S.
dc.contributor.authorPakingking Jr., Rolando V.
dc.date.accessioned2017-09-12T07:03:41Z
dc.date.available2017-09-12T07:03:41Z
dc.date.issued2017
dc.identifier.citationAmar, E. C., Faisan Jr., J. P., Apines-Amar, M. J. S., & Pakingking Jr., R. V. (2017). Temporal changes in innate immunity parameters, epinecidin gene expression, and mortality in orange-spotted grouper, Epinephelus coioides experimentally infected with a fish pathogen, Vibrio harveyi JML1. Fish & Shellfish Immunology, 69, 153-163.en
dc.identifier.issn1050-4648
dc.identifier.urihttp://hdl.handle.net/10862/3220
dc.description.abstractChanges in innate immunity parameters and epinecidin mRNA transcript levels were examined to characterize the non-specific immune response of E. coioides to pathogenic V. harveyi JML1 isolated from affected cage-cultured fish. After fish had been injected with bacteria at a dose causing 30% mortality, blood and tissue samples were collected at 0, 6, 12, 24, 48, 72, 96, 120, and 240 h post-infection (hpi) for assessment of indices such as the oxidative burst (OB) and phagocytic index (PI) of head kidney cells, and lysozyme activity (LYS) and total immunoglobulin (Total Ig) levels of the plasma. The epinecidin mRNA transcript levels (EGE) from skin, gills, liver, kidney, and spleen tissues were also determined by gelbased RT-PCR. Lastly, daily mortality (DM), liver total bacterial load (TBC), and presumptive Vibrio count (TVC) were monitored up to 240 hpi. The results revealed that bacteria proliferated rapidly in fish tissue, reaching peak densities at 24 hpi for both TBC and TVC but was on a downward trend thereafter. The pattern in fish mortality closely correlated with TBC and TVC. Total Ig, OB, and PI in E. coioides were suppressed in the early part of infection when V. harveyi load was high but recovered and later increased as bacterial density declined. LYS and EGE were consistently high and their activities were not hampered by bacterial infection. The study demonstrated that V. harveyi JML1 interacts with E. coioides by transiently inhibiting some immune parameters resulting in mortalities. However, consistently high LYS, upregulated EGE, and resurgent PI, OB and Total Ig conferred resistance and subsequent recovery in the fish. The study provides new insights on the interaction between E. coioides and V. harveyi JML1 that can aid in formulating health management strategies for groupers. Further studies on prophylactic interventions to enhance the innate immune response in grouper during infection with V. harveyi JML1 are suggested.en
dc.description.sponsorshipThis study was supported by SEAFDEC/AQD under Study Code 5203-T-RD-FH0510. The assistance of the Fish Health Section Staff is gratefully acknowledged.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectImmune responseen
dc.subjectHost pathogen relationsen
dc.subjectHost-pathogen interactionen
dc.subjectEpinecidin gene expressionen
dc.subjectEpinephelus coioides
dc.subjectVibrio harveyi
dc.titleTemporal changes in innate immunity parameters, epinecidin gene expression, and mortality in orange-spotted grouper, Epinephelus coioides experimentally infected with a fish pathogen, Vibrio harveyi JML1en
dc.typeArticleen
dc.citation.volume69
dc.citation.spage153
dc.citation.epage163
dc.citation.journalTitleFish and Shellfish Immunologyen
dc.subject.asfaImmunityen
dc.subject.asfaPathogensen
dc.subject.asfaHostsen
dc.subject.asfaPathogenic bacteriaen
dc.subject.asfaDisease resistanceen
dc.subject.asfaGene expressionen
dc.identifier.doi10.1016/j.fsi.2017.08.005


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  • AQD Journal Articles [1136]
    These papers were contributed by AQD staff to various national and international journals

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