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dc.contributor.authorTan-Fermin, J. D.
dc.contributor.authorGapasin, R. S. J.
dc.contributor.authorTan, A. M.
dc.contributor.authorGarcia, M. A.
dc.contributor.authorEmata, A. C.
dc.contributor.editorMarte, Clarissa L.
dc.contributor.editorQuinitio, Gerald F.
dc.contributor.editorEmata, Arnil C.
dc.date.accessioned2011-08-05T09:28:01Z
dc.date.available2011-08-05T09:28:01Z
dc.date.issued1996
dc.identifier.citationTan-Fermin, J. D. (1996). LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther). In C. L. Marte, G. F. Quinitio, & A. C. Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 (p. 110). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511326
dc.identifier.urihttp://hdl.handle.net/10862/569
dc.descriptionAbstract only.en
dc.description.abstractClarias macrocephalus is endemic yet dwindling freshwater foodfish in the Philippines. Induced breeding protocol was developed by monitoring the size and maturation of eggs at 0-48 h after a simultaneous injection of luteinizing hormone-releasing hormone analogue (LHRHa; 0.0005 - 0.10 µg/g BW) and pimozide (PIM; 1 µg/g BW). Based on its similar osmotic pressure with catfish plasma, eggs were fixed in 1% phosphate-buffered formalin. Mean egg diameter of fish that were induced to mature increased during ovulation. Oocyte maturation, indicated by oocytes with germinal vesicle breakdown (GVBD), was observed at least 12 h post-injection in fish given 0.01 - 0.10 µg LHRHa + 1 µg PIM/g BW, followed by ovulation 4 h thereafter. Results showed that a simultaneous injection of C. macrocephalus with 0.05 µg LHRHa + 1 µg PIM/g BW at 1800-1900 h followed by stripping at 16-20 h post-injection resulted in high ovulation, fertilization and hatching rates.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectClarias macrocephalus
dc.titleLHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther)en
dc.typeConference paperen
dc.citation.spage110
dc.subject.asfaEndemic speciesen
dc.subject.asfaOvulationen
dc.subject.asfaSexual maturityen
dc.subject.asfaFish eggsen
dc.subject.asfaOocytesen
dc.subject.asfaStandardsen
dc.subject.asfaInduced breedingen
dc.subject.asfaFreshwater fishen
dc.subject.asfaBiological fertilizationen
dc.subject.asfaHatchingen
dc.subject.asfaOogenesisen
dc.subject.asfaSex hormonesen
dc.citation.conferenceTitleProceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993en


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