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dc.contributor.authorRuangpan, Lila
dc.date.accessioned2013-09-08T14:16:09Z
dc.date.available2013-09-08T14:16:09Z
dc.date.issued2004
dc.identifier.citationRuangpan, L. (2004). Minimal inhibitory concentration (MIC) test and determination of antimicrobial resistant bacteria. In Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment (pp.31-55). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511741
dc.identifier.urihttp://hdl.handle.net/10862/1637
dc.description.abstractThe agar dilution technique is used to measure qualitatively the in vitro activity of an antimicrobial agent against the test bacteria. In this method, graded amounts of antibiotics are incorporated in agar plates and inoculated in spots with the organisms under study. If the organism under study is susceptible to the incorporated antibiotic, no bacterial growth is expected in agar plates with higher amounts of the drugs. Bacterial growth is observed as the antibiotic concentration in the agar plate diminishes. Inhibition of growth at the minimum or lowest concentration of antibiotic is regarded as the end point.en
dc.description.sponsorshipThe Government of Japan Trust Fund through SEAFDEC Aquaculture Department provided financial support for the research project and publication of this manual. We would like to express our sincere thanks to Dr. Yasuo Inui and Dr. Kazuya Nagasawa, the former and current Fish Disease Experts and Leaders of the Regional Fish Disease Project, for their encouragement and critical suggestions; and the staff of the Fish Health Section, SEAFDEC Aquaculture Department, for their kind cooperation, especially to Dr. Celia R. Lavilla-Pitogo for carefully reading the manuscript and Dr. Edgar C. Amar for serving as the over-all coordinator in the preparation of drafts. Thanks also to Dr. Hisatsugu Wakabayashi, Emeritus Professor of Fish Pathology Laboratory at the University of Tokyo, for reviewing the manuscript.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.relation.ispartofRuangpan, L., & Tendencia, E. A. (2004). Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Aquaculture Department, Southeast Asian Fisheries Development Center. http://hdl.handle.net/10862/1615en
dc.titleMinimal inhibitory concentration (MIC) test and determination of antimicrobial resistant bacteriaen
dc.typeBook chapteren
dc.citation.spage31
dc.citation.epage55
dc.subject.asfaagaren
dc.subject.asfagrowthen
dc.subject.asfatestsen
dc.subject.asfastandardsen
dc.subject.asfaantibioticsen
dc.subject.asfabacteriologyen
dc.subject.asfamicrobiological analysisen
dc.subject.asfamethodologyen
dc.citation.bookTitleLaboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environmenten


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