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dc.contributor.authorChieu, Hoang Dinh
dc.contributor.authorTurner, Luke
dc.contributor.authorSmith, Meaghan K.
dc.contributor.authorWang, Tianfang
dc.contributor.authorNocillado, Josephine
dc.contributor.authorPalma, Peter
dc.contributor.authorSuwansa-ard, Saowaros
dc.contributor.authorElizur, Abigail
dc.contributor.authorCummins, Scott F.
dc.date.accessioned2020-07-14T08:39:31Z
dc.date.available2020-07-14T08:39:31Z
dc.date.issued2019-02-19
dc.identifier.citationChieu, H. D., Turner, L., Smith, M. K., Wang, T., Nocillado, J., Palma, P., Suwansa-ard, S., Elizur, A., & Cummins, S. F. (2019). Aquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptide. Frontiers in Genetics, 10, 77.en
dc.identifier.urihttp://hdl.handle.net/10862/5902
dc.descriptionThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fgene.2019.00077/full#supplementary-materialen
dc.description.abstractWild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea cucumber reproductive processes. The neurohormone relaxin-like gonad-stimulating peptide (RGP) has been identified as the active gonad-stimulating peptide in sea stars (Asteroidea), which could also be present in other echinoderm groups. In this study, a sea cucumber RGP was identified and confirmed by phylogenetic analysis. A recombinant Holothuria scabra RGP was produced in the yeast Pichia pastoris and confirmed by mass spectrometry. To assess bioactivity, four levels of purification were tested in an in vitro germinal vesicle breakdown (GVBD) bioassay. The most pure form induced 98.56 ± 1.19% GVBD in H. scabra and 89.57 ± 1.19% GVBD in Holothuria leucospilota. Cruder levels of purification still resulted in some GVBD. Upon single injection into female H. scabra, the recombinant RGP induced head waving behavior followed by spawning within 90–170 min. Spawned oocytes were fertilized successfully, larvae settled and developed into juveniles. Our results provide a key finding for the development of a break-through new artificial breeding approach in sea cucumber aquaculture.en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fgene.2019.00077/pdfen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectsea cucumbersen
dc.subjectHolothuria scabraen
dc.subjectHolothuria leucospilotaen
dc.titleAquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptideen
dc.typeArticleen
dc.citation.volume10
dc.citation.spage77
dc.citation.journalTitleFrontiers in Geneticsen
dc.subject.asfaaquacultureen
dc.subject.asfajuvenilesen
dc.subject.asfaoocytesen
dc.subject.asfagenetic processesen
dc.subject.asfaspawningen
dc.identifier.essn1664-8021
dc.identifier.doi10.3389/fgene.2019.00077
dc.subject.scientificNameHolothuria scabra
dc.subject.scientificNamePichia pastoris
dc.subject.scientificNameHolothuria leucospilota
local.subjectaquacultureen
local.subjectjuvenileen
local.subjectoocyte maturationen
local.subjectrecombinant RGPen
local.subjectsea cucumbersen
local.subjectspawningen


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    These papers were contributed by AQD staff to various national and international journals

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