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dc.contributor.authorMandal, Anup
dc.contributor.authorMani, Anjali Kottayil
dc.contributor.authorLamech, Ruban
dc.contributor.authorAnandajothi, Elamaran
dc.contributor.authorVenkatachalam, Shanmuga Arasu
dc.contributor.authorDinakaran, Gajendiran K.
dc.contributor.authorQuinitio, Emilia T.
dc.contributor.authorKandan, Shanmuganathan
dc.date.accessioned2021-02-26T00:55:32Z
dc.date.available2021-02-26T00:55:32Z
dc.date.issued2021-02-05
dc.identifier.citationMandal, A., Mani, A. K., Lamech, R., Anandajothi, E., Venkatachalam, S. A., Dinakaran, G. K., Quinitio, E. T., & Kandan, S. (2021). Genetic identification of all four mangrove mud crab species (genus Scylla) using multiple molecular markers. Biochemical Genetics. https://doi.org/10.1007/s10528-021-10032-3en
dc.identifier.issn0006-2928
dc.identifier.urihttp://hdl.handle.net/10862/6032
dc.description.abstractMisleading identification and subsequent publications on biological, molecular, and aquaculture data of mangrove mud crab (genus Scylla de Hann 1833) is a major concern in many countries. In this study, multiple molecular markers were used for genetic identification of all four known mud crab species under genus Scylla collected from India, Philippines, Myanmar, Malaysia and Indonesia. Internal Transcribed Spacer (ITS-1), Polymerase chain reaction (PCR)–Restriction Fragment Length Polymorphism (PCR–RFLP) and PCR-based species-specific markers were used to resolve taxonomic ambiguity. PCR–RFLP techniques using NlaIV and BsaJI restriction endonucleases were efficient to differentiate four different mud crab species under genus Scylla with specific fragment profile. The results also justified the use of ITS-1 and PCR-based species-specific markers to identify mud crab species available in many countries quite rapidly and effectively. Several new molecular markers generated during the study are reported here to resolve the taxonomic ambiguity of Scylla species and the results reconfirmed that India is only having two commonly available mud crab species which was reported by the authors earlier.en
dc.language.isoenen
dc.publisherSpringeren
dc.subjectScyllaen
dc.subjectTaxonomic ambiguityen
dc.subjectcrabsen
dc.titleGenetic identification of all four mangrove mud crab species (genus Scylla) using multiple molecular markersen
dc.typeArticleen
dc.citation.volume59en
dc.citation.spage856en
dc.citation.epage869en
dc.citation.journalTitleBiochemical Geneticsen
dc.subject.asfaspecies identificationen
dc.subject.asfagenetic markersen
dc.subject.asfamolecular markersen
dc.subject.asfacrab cultureen
dc.subject.asfaDNAen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfacytochromesen
dc.subject.asfasequencingen
dc.subject.asfadata processingen
dc.subject.asfaenzymesen
dc.subject.asfataxonomyen
dc.identifier.essn1573-4927
dc.identifier.doi10.1007/s10528-021-10032-3


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  • AQD Journal Articles [1213]
    These papers were contributed by AQD staff to various national and international journals

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