dc.contributor.author | Formacion, Minda J. | |
dc.contributor.author | Lam, Tom J. | |
dc.contributor.editor | Marte, Clarissa L. | |
dc.contributor.editor | Quinitio, Gerald F. | |
dc.contributor.editor | Emata, Arnil C. | |
dc.date.accessioned | 2011-08-05T09:28:05Z | |
dc.date.available | 2011-08-05T09:28:05Z | |
dc.date.issued | 1996 | |
dc.identifier.citation | Formacion, M. J., & Lam, T. J. (1996). Endocrine changes associated with overripening of ovulated eggs in goldfish, Carassius auratus L. In C. L. Marte, G. F. Quinitio, & A. C. Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 (pp. 89-96). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. | en |
dc.identifier.isbn | 9718511326 | |
dc.identifier.uri | http://hdl.handle.net/10862/591 | |
dc.description.abstract | Changes in steroid hormone levels in the serum and ovarian fluid were studied during overripening in goldfish. Ovulated eggs retained in the ovarian cavity become overripe at around 12 h after ovulation and completely overripe 24 h after. Blood and ovarian fluid were taken at 0, 3, 6, 12, 18, and 24 h after ovulation. Estradiol-17ß (E 2) , testosterone (T), progesterone (P) and 17α,20ß-dihydroxy-4-pregnen-3-one (17α,20ß-P) in the serum were extracted directly with a solvent while those in the ovarian fluid were separated by HPLC before radioimmunoassay. Both serum and ovarian fluid P showed a highly significant decline at 18 h with a further decline at 24 h; P levels were higher in the ovarian fluid. Serum 17α,20ß-P showed a progressive and more rapid decline, decreasing significantly at 12 h with further decreases at 18 h and 24 h; the level was five-fold lower at 24 h compared to the 0 h level. Serum T increased significantly at 3 h which was maintained until 18 h, when it declined to 0 h level. No significant changes in E2 were observed in the serum, except for a significant difference between 6 and 24 h. There were no significant changes in E2, T and 17α,20ß-P in the ovarian fluid. Of the four steroids measured, only 17α,20ß-P and P showed changes which bear some correlation with the time course of overripening. The declines in the mean ratios of 17α,20ß-P/E2 in the serum and P/E2 in the ovarian fluid also appeared to have a good correlation with the time course of overripening. The postovulatory follicles (POFs) showed degenerative features which likewise correspond to the decline in P and 17α,20ß-P. | en |
dc.language.iso | en | en |
dc.publisher | Aquaculture Department, Southeast Asian Fisheries Development Center | en |
dc.subject | Carassius auratus | |
dc.subject | Goldfish | en |
dc.title | Endocrine changes associated with overripening of ovulated eggs in goldfish, Carassius auratus L. | en |
dc.type | Conference paper | en |
dc.citation.spage | 89 | |
dc.citation.epage | 96 | |
dc.subject.asfa | HPLC | en |
dc.subject.asfa | ovulation | en |
dc.subject.asfa | endocrine glands | en |
dc.subject.asfa | fish eggs | en |
dc.subject.asfa | Ornamental fish | en |
dc.subject.asfa | ovaries | en |
dc.subject.asfa | Freshwater fish | en |
dc.subject.asfa | steroids | en |
dc.subject.asfa | Immunoassays | en |
dc.subject.asfa | sex hormones | en |
dc.citation.conferenceTitle | Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 | en |