SEAFDEC/AQDhttp://hdl.handle.net/20.500.12066/39722024-03-28T14:17:18Z2024-03-28T14:17:18ZPromotion of sustainable aquaculture, aquatic animal health, and resource enhancement in Southeast Asia: Proceedings of the international workshop on the promotion of sustainable aquaculture, aquatic animal health, and resource enhancement in Southeast Asia, 25–27 June 2019, Iloilo City, Philippineshttp://hdl.handle.net/20.500.12066/69042022-03-16T08:57:02Z2021-12-01T00:00:00ZPromotion of sustainable aquaculture, aquatic animal health, and resource enhancement in Southeast Asia: Proceedings of the international workshop on the promotion of sustainable aquaculture, aquatic animal health, and resource enhancement in Southeast Asia, 25–27 June 2019, Iloilo City, Philippines
Aya, Frolan A.; de la Peña, Leobert D.; Salayo, Nerissa D.; Tendencia, Eleonor A.
This publication is a product of the workshop and compiles information from the thirty presentations made during the event. Reports from member countries, invited experts, and SEAFDEC/AQD projects supported by the GOJ-TF on the topics of sustainable aquaculture, resource enhancement, and aquatic animal health are bared in these pages. The workshop summary, synthesizing the key points raised during the workshop, should be a valuable reference on the broad challenges we face, as well as the solutions and actions that must be done.
2021-12-01T00:00:00ZFactors affecting mortality of shrimp, Penaeus monodon, experimentally infected with Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VPAHPND)Tendencia, EleonorQuitor, Geraldine C.http://hdl.handle.net/20.500.12066/68802022-03-16T08:50:38Z2021-12-01T00:00:00ZFactors affecting mortality of shrimp, Penaeus monodon, experimentally infected with Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VPAHPND)
Tendencia, Eleonor; Quitor, Geraldine C.
Aya, Frolan A.; de la Peña, Leobert D.; Salayo, Nerissa D.; Tendencia, Eleonor A.
One of the most recent diseases affecting the shrimp industry is the early mortality syndrome (EMS). EMS, characterized by observed mortality in shrimp within the first 35 days of culture, is due to several diseases, one of which is the acute hepatopancreatic disease (AHPND). Outbreaks due to AHPND have caused economic losses to many shrimp producing countries globally. This paper investigates factors affecting mortality of shrimp, Penaeus monodon experimentally infected with Vibrio parahaemolyticus causing AHPND (VP<sub>AHPND</sub>).
Tank experiments done suggested that exposure to 10<sup>7</sup> cfu/ml VP<sub>AHPND</sub>, 35°C, and 10 and 28 ppt increase the risk of shrimp mortality due to AHPND. The VP<sub>AHPND</sub> concentration in the water that P. monodon can overcome is <105 cfu/ml. Observed mortality due to VP<sub>AHPND</sub> is age related, with higher mortalities in younger infected shrimp.
2021-12-01T00:00:00ZAcute toxicity of garlic (Allium sativum) extract to snubnose pompano (Trachinotus blochii) juvenileErazo-Pagador, Gregoriahttp://hdl.handle.net/20.500.12066/68772022-03-16T08:49:41Z2021-12-01T00:00:00ZAcute toxicity of garlic (Allium sativum) extract to snubnose pompano (Trachinotus blochii) juvenile
Erazo-Pagador, Gregoria
Aya, Frolan A.; de la Peña, Leobert D.; Salayo, Nerissa D.; Tendencia, Eleonor A.
Garlic (Allium sativum) is a well-known medicinal herb which has been shown to possess anti-microbial and anti-parasitic properties. This study was conducted to test the toxicity levels of snubnose pompano (Trachinotus blochii) juvenile to garlic (Allium sativum) extract by determining the cumulative mortality and median lethal concentration (LC50). Test fish were exposed to six concentrations of the extract (2.5, 5.0, 7.5, 10, 20 and 30 ppm) in a 96-hour static bioassay. Cumulative mortality was highest at 100 % for 30 ppm garlic extract, with mortalities found to increase with increasing concentration. Test fish exposed to 20 and 30 ppm exhibited weak and static behavior. The LC50 of garlic extract to T. blochii was found to be 7.48 ppm at 96 h. Findings of the present study suggest that aqueous garlic extract up to 5 ppm can be safely used in pompano for prophylactic purposes.
2021-12-01T00:00:00ZApplication of carriers and RNAi to enhance the antiviral immune response of shrimp to WSSVAmar, EdgarBaes, CharisSuperio, JoshuaSomera, Mechil D.Cordero, Christianhttp://hdl.handle.net/20.500.12066/69002022-03-16T08:55:40Z2021-12-01T00:00:00ZApplication of carriers and RNAi to enhance the antiviral immune response of shrimp to WSSV
Amar, Edgar; Baes, Charis; Superio, Joshua; Somera, Mechil D.; Cordero, Christian
Aya, Frolan A.; de la Peña, Leobert D.; Salayo, Nerissa D.; Tendencia, Eleonor A.
In aquaculture, vaccination is one of the approaches for disease prevention and control. The aim of the present study was to determine the efficacy of a VP28 double stranded RNA (VP28 dsRNA) and recombinant VP28 protein (rVP28) administered together as an antiviral treatment against WSSV. Double-stranded RNA was produced in RNAsedeficient Escherichia coli HT115 following published methods. To determine the appropriate dose, different concentrations of dsRNA ranging between 0.2 μg and 20 μg, were either injected intramuscularly or delivered orally to the shrimp via the feed ration. Thereafter, the shrimp were challenged with WSSV either by injection (LD<sub>50</sub>=10<sup>-7</sup> dilution of the gill tissue filtrate) or bath immersion (LD<sub>50</sub>=10<sup>-4</sup> dilution of the filtrate) in glass aquaria and transferred to fiberglass tanks for daily monitoring and recording of mortalities. Results showed significant differences in survival between PBS and the 0.2, 10, and 20 μg dsRNA/shrimp doses. Time to 100 % mortality significantly differed among the treatments with the control reaching mortality earlier (day 4) while shrimp injected with 0.2 and 10 μg dsRNA succumbed to WSSV much later on days 9–12. Different frequencies of dsRNA administration were also tested. The best result obtained was a dose of 20 μg/shrimp administered 4 times over 28 days (2 times before and 2 times during challenge for a total 80 μg/shrimp). Finally, VP28 dsRNA was combined with rVP28 at ratios of 1:1, 1:2, 1:3, 3:1, and 2:1, entrapped in chitosan microparticles and delivered per os via the feed according to the dose and frequency as previously determined. Following bath exposure challenge with WSSV, the best survival obtained in trials 1 and 2 was 40 % and 43 % at 1:3 VP28 dsRNA to rVP28 ratio.
2021-12-01T00:00:00Z