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dc.contributor.authorGarcia, Luis Maria
dc.date.accessioned2013-01-14T08:05:35Z
dc.date.available2013-01-14T08:05:35Z
dc.date.issued1989
dc.identifier.citationGarcia, L. M. B. (1989). Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch). Aquaculture, 77(1), 97–102.en
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/1297
dc.description.abstractA convenient and rapid biopsy method for taking ovarian samples from mature sea bass (Lates calcarifer Bloch) is described. Intra-ovarian oocytes siphoned with polyethylene tubing from any region of the ovary provide a sample representative of the maturational stage of sea bass. The osmolality of a 5% phosphate buffered formalin solution is similar to that of sea bass plasma. The follicular diameter of cannulated sea bass oocytes can be measured within an hour after fixation in 5% phosphate buffered formalin without significantly deviating from the diameter of fresh oocytes.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectLates calcariferen
dc.subjectbiopsyen
dc.subjectsea bassen
dc.titleDevelopment of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).en
dc.typeArticleen
dc.citation.volume77
dc.citation.issue1
dc.citation.spage97
dc.citation.epage102
dc.citation.journalTitleAquacultureen
dc.subject.asfamethodologyen
dc.subject.asfasexual maturityen
dc.subject.asfaovariesen
dc.identifier.doi10.1016/0044-8486(89)90025-2
dc.subject.scientificNameLates calcariferen


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