Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines
Cited times in Scopus
Viral nervous necrosis (VNN), also known as viral encephalopathy and retinopathy (VER), is an emerging disease affecting larvae and juveniles of many farmed marine fish species in Asia, Australia, Europe and North America. Mass mortality occurred in 14-day old larval sea bass Lates calcarifer at a hatchery in the Philippines associated with clinical signs such as abnormal swimming behavior and pale-gray discoloration of the body. Histological investigations in moribund fish revealed marked vacuolation in the retina and brain. Cytopathic effects (CPE) were observed in SSN-1 cells inoculated with the tissue filtrate of affected sea bass. A piscine nodavirus, the causative agent of VNN, was detected in the affected tissues and SSN-1 cells inoculated with the tissue filtrate of affected fish by RT-PCR. Electron microscopy revealed non-enveloped viral particles, 22-28 nm in diameter, in the cytoplasm of the brain and retina of affected fish and in the cytoplasm of VNN-infected SSN-1 cells after CPE appeared. These results indicate that mass mortality of sea bass larvae in the Philippines was caused by a piscine nodavirus.
Suggested CitationMaeno, Y., de la Peña, L. D., & Cruz-Lacierda, E. R. (2004). Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines.
- AQD Journal Articles 
Showing items related by title, author, creator and subject.
Bagarinao, Teodora; Kungvankij, Pinij (Elsevier, 1986)This paper describes an incidence of swimbladder malfunction causing high positive buoyancy and mass mortality in 2-week-old sea bass (Lates calcarifer) larvae reared in an outdoor hatchery tank under conditions of high ...
The ultrastructure of the hepatocytes of the giant seaperch, Lates calcarifer (Bloch) (Pisces: Centropomidae), during starvation and refeeding with different diets. Avila, Enrique M. (The Marine Biological Association of Hongkong, 1986)Three groups of immature seaperch acclimated in the laboratory on a mixed commercial pellet and minced trashfish diet were starved for 30 days. Thereafter, the first group was starved for 7 more days, the second was refed ...
Improved larval survival at metamorphosis of Asian seabass (Lates calcarifer) using ω3-HUFA-enriched live food Dhert, Philippe; Lavens, Patrick; Duray, Marietta; Sorgeloos, Patrick (Elsevier, 1990)Asian seabass (Lates calcarifer) larvae were fed Brachionus cultured on Chlorella and, as soon as ingestin was possible, different types of Artemia, i.e., nauplii of the San Francisco Bay (SFB) strain, Great Salt Lake (GSL) ...