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dc.contributor.authorLazarte, Jassy Mary S.
dc.contributor.authorManingas, Mary Beth B.
dc.contributor.editorRomana-Eguia, Maria Rowena R.
dc.contributor.editorParado-Estepa, Fe D.
dc.contributor.editorSalayo, Nerissa D.
dc.contributor.editorLebata-Ramos, Ma. Junemie Hazel
dc.date.accessioned2016-01-20T07:40:21Z
dc.date.available2016-01-20T07:40:21Z
dc.date.issued2015
dc.identifier.citationLazarte, J. M. S., &Maningas, M. B. B. (2015). Targeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSV. In M. R. R. Romana-Eguia, F. D. Parado-Estepa, N. D. Salayo, &M. J. H. Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA) (pp. 283-298). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9789719931041
dc.identifier.urihttp://hdl.handle.net/10862/2783
dc.description.abstractThe white spot syndrome virus (WSSV) remains to be the most widespread and devastating infectious agent that has hit particularly the marine shrimp aquaculture industry worldwide. To date, there are no known effective strategies that can combat WSSV infection. This study aimed to elucidate host-pathogen interaction through the functional study of host - gene. Utilizing RNA Interference, the function of contig23 (c23) in the shrimp genome, identified to have high homology with WSSVORF-325, was determined. Three set-ups were prepared for treatment of c23-, GFP-dsRNA, and PBS using Macrobrachium rosenbergii freshwater prawns. Each treatment group was challenged with WSSV and survival rate was recorded. C23-, and GFP-dsRNA injected prawns showed a significant survival rate of 100%, in contrast to 20% of the PBS injected prawns at 10 days post-infection (dpi). Results showed that injection of c23- and GFP-dsRNA prior to challenge with WSSV, delayed and reduced mortality in contrast to PBS-treated prawns, which showed high mortality. Gene expression analysis showed silencing of both WSSV and c23 at day 3 post-WSSV challenge. This study proved that c23-dsRNA has a protective effect on WSSVchallenged prawns and highlights its involvement in the infectivity of WSSV in M. rosenbergii.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectMacrobrachium rosenbergii
dc.subjectWSSVen
dc.subjectHost-pathogen infectionen
dc.subjectShrimp genomeen
dc.subjectGene expression analysisen
dc.titleTargeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSVen
dc.typeConference paperen
dc.citation.spage283
dc.citation.epage298
dc.subject.asfagenomesen
dc.subject.asfamortalityen
dc.subject.asfaviral diseasesen
dc.subject.asfashrimp cultureen
dc.subject.asfaRNAen
dc.subject.asfafish diseasesen
dc.subject.asfahusbandry diseasesen
dc.subject.asfadisease prophylaxisen
dc.subject.asfasurvivalen
dc.subject.asfaprawn cultureen
dc.subject.asfapathogensen
dc.citation.conferenceTitleResource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA)en


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