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dc.contributor.authorde la Peña, Leobert D.
dc.contributor.editorLio-Po, Gilda D.
dc.contributor.editorLavilla, Celia R.
dc.contributor.editorCruz-Lacierda, Erlinda R.
dc.date.accessioned2011-10-29T09:45:18Z
dc.date.available2011-10-29T09:45:18Z
dc.date.issued2001
dc.identifier.citationde la Peña, L. D. (2001). Immunological and molecular biology techniques in disease diagnosis. In G. D. Lio-Po, C. R. Lavilla, & E. R. Cruz-Lacierda (Eds.), Health Management in Aquaculture (pp. 137-158). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511458
dc.identifier.urihttp://hdl.handle.net/10862/733
dc.description.abstractThe improvement of existing immunoassay techniques, development of monoclonal antibody technology and the development of new immunoassay approaches are all working together to provide new tools for the detection of disease-causing organisms in fish and crustaceans. Following the introduction of nucleic acid hybridization technique and PCR, it was recognized that the methods offered a sensitive approach to the detection and identification of specific microorganisms as in the case of a bacterial or viral infection in a variety of sample types. Potentially, a characteristic DNA sequence from a single virus particle or cell of a particular organism can be amplified to detectable levels within a short period of time. Conventional diagnostic methods that involve the culture of microorganisms can take days or weeks to complete or very tedious to perform. PCR offers a rapid, very sensitive, very specific and simple alternative. Further developments in immunodiagnostics and emerging technologies such as DNA-based tests will revolutionize the detection and identification of infectious disease agents.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectPhilippinesen
dc.subjectAsiaen
dc.titleImmunological and molecular biology techniques in disease diagnosisen
dc.typeBook chapteren
dc.citation.spage137
dc.citation.epage158
dc.subject.asfaviral diseasesen
dc.subject.asfashrimp cultureen
dc.subject.asfafish diseasesen
dc.subject.asfamonoclonal antibodiesen
dc.subject.asfahusbandry diseasesen
dc.subject.asfabacterial diseasesen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfaDisease detectionen
dc.subject.asfaImmunoassaysen
dc.subject.asfafish cultureen
dc.citation.bookTitleHealth Management in Aquacultureen


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