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  • Breeding and Seed Production of Cultured Finfishes in the Philippines
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  •   SEAFDEC Institutional Repository (SIR)
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  • 02 Technical Publications and Reports on Aquaculture
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  • Breeding and Seed Production of Cultured Finfishes in the Philippines
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LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther)

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bspcfp_p110a.pdf (42.22Kb)
Date
1996
Author
Tan-Fermin, J. D.
Gapasin, R. S. J.
Tan, A. M.
Garcia, M. A.
Emata, Arnil C.
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Abstract
Clarias macrocephalus is endemic yet dwindling freshwater foodfish in the Philippines. Induced breeding protocol was developed by monitoring the size and maturation of eggs at 0-48 h after a simultaneous injection of luteinizing hormone-releasing hormone analogue (LHRHa; 0.0005 - 0.10 µg/g BW) and pimozide (PIM; 1 µg/g BW). Based on its similar osmotic pressure with catfish plasma, eggs were fixed in 1% phosphate-buffered formalin. Mean egg diameter of fish that were induced to mature increased during ovulation. Oocyte maturation, indicated by oocytes with germinal vesicle breakdown (GVBD), was observed at least 12 h post-injection in fish given 0.01 - 0.10 µg LHRHa + 1 µg PIM/g BW, followed by ovulation 4 h thereafter. Results showed that a simultaneous injection of C. macrocephalus with 0.05 µg LHRHa + 1 µg PIM/g BW at 1800-1900 h followed by stripping at 16-20 h post-injection resulted in high ovulation, fertilization and hatching rates.
Description
Abstract only.
URI
http://hdl.handle.net/10862/569
Suggested Citation
Tan-Fermin, J. D. (1996). LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther). In C. L. Marte, G. F. Quinitio, & A. C. Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 (p. 110). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Subject
endemic species ASFA; ovulation ASFA; sexual maturity ASFA; fish eggs ASFA; oocytes ASFA; standards ASFA; induced ovulation ASFA; Freshwater fish; Biological fertilization; hatching ASFA; oogenesis ASFA; sex hormones ASFA; Clarias macrocephalus
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  • Breeding and Seed Production of Cultured Finfishes in the Philippines [31]

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