Bacterial isolation, identification and storage
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Bacterial isolation, purification and identification are the first steps to bacteriological studies. Isolation is done to obtain pure bacterial cultures. Bacteria are usually isolated from fish kidney and spleen; and from the hepatopancreas, lymphoid organ and muscles of shrimp. These tissues are monitor organs that usually harbor the disease-causing bacteria during infection.
Ruangpan, L., & Tendencia, E. A. (2004). Bacterial isolation, identification and storage. In Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment (pp. 3–11). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.