dc.contributor.author | Pedroso, Fiona L. | |
dc.contributor.author | De Jesus-Ayson, Evelyn Grace T. | |
dc.contributor.author | Cortado, Hanna H. | |
dc.contributor.author | Hyodo, Susumu | |
dc.contributor.author | Ayson, Felix G. | |
dc.date.accessioned | 2011-06-01T09:49:36Z | |
dc.date.available | 2011-06-01T09:49:36Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Pedroso, F. L., de Jesus-Ayson, E. G. T., Cortado, H. H., Hyodo, S., & Ayson, F. G. (2006). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status. General and Comparative Endocrinology, 145(3), 237-246. | en |
dc.identifier.issn | 0016-6480 | |
dc.identifier.uri | http://hdl.handle.net/10862/94 | |
dc.description.abstract | Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the cDNAs for grouper GH and IGF-I and examined their mRNA expression during different nutritional status. Grouper GH cDNA is 936 base pairs (bp) long excluding the poly-A tail. It contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. It has an open reading frame of 612bp coding for a signal peptide of 17 amino acids (aa) and a mature hormone of 187aa residues. Based on the aa sequence of the mature hormone, grouper GH shows higher sequence identity (>76%) to GHs of perciforms than to GHs of cyprinids and salmonids (53-69%). Grouper preproIGF-I cDNA consisted of 558bp, which codes for 186aa. This is composed of 44aa for the signal peptide, 68aa for the mature peptide comprising B, C, A, and D domains, and 74aa for the E domain. Mature grouper IGF-I shows very high sequence identity to IGF-I of teleost fishes (84-97%) compared to advanced groups of vertebrates such as chicken, pig, and human (=<80%). Using DNA primers specific for grouper GH and IGF-I, the changes in mRNA levels of pituitary GH and hepatic IGF-I in response to starvation and refeeding were examined by a semi-quantitative RT-PCR. Significant elevation of GH mRNA level was observed after 2 weeks of food deprivation, and increased further after 3 and 4 weeks of starvation. GH mRNA level in fed-controls did not change significantly during the same period. Hepatic IGF-I mRNA level decreased significantly starting after 1 week of starvation until the 4th week. There was no significant change in IGF-I mRNA levels in fed-controls. One week of refeeding can restore the GH and IGF-I mRNA back to its normal levels. Deprivation of food for 1-4 weeks also resulted in cessation of growth and decrease in condition factor. | en |
dc.description.sponsorship | We thank Jossette Bangcaya and Josephine Nocillado for providing us degenerate IGF-I reverse primer, Mr. Roman Sanares for the statistical analyses, Dr. Ebonia Seraspe for her valuable advice and help. This study was funded by SEAFDEC AQD to EGT de Jesus-Ayson (Nr-01-F2001T) and by a grant from USAID to FG Ayson (Grant No. TA-MOU-99-C19-001, Program in US-Israel Cooperative Development Research, Economic Growth). | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.ispartof | General and Comparative Endocrinology 145:237-246 | en |
dc.subject | somatotropin | en |
dc.subject | insulin-like growth factor | en |
dc.subject | nutritional status | en |
dc.subject | open reading frames | en |
dc.title | Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status | en |
dc.type | Article | en |
dc.citation.volume | 145 | |
dc.citation.issue | 3 | |
dc.citation.spage | 237 | |
dc.citation.epage | 246 | |
dc.citation.journalTitle | General and Comparative Endocrinology | en |
dc.subject.asfa | hormones | en |
dc.subject.asfa | starvation | en |
dc.subject.asfa | nutrition | en |
dc.subject.asfa | endocrinology | en |
dc.subject.asfa | growth rate | en |
dc.subject.asfa | food availability | en |
dc.subject.asfa | marine fishes | en |
dc.subject.asfa | condition factor | en |
dc.subject.asfa | gene expression | en |
dc.subject.asfa | liver | en |
dc.subject.asfa | amino acids | en |
dc.subject.asfa | polymerase chain reaction | en |
dc.subject.asfa | primers | en |
dc.identifier.doi | 10.1016/j.ygcen.2005.09.001 | |