Clonal production of Kappaphycus alvarezii (Doty) Doty in vitro
រកមើល / បើកOpen
Request this document
កាលបរិច្ឆេទ
2017Page views
204ទិន្នន័យមេតា
មើលកំណត់ត្រាលម្អិតនៃធាតុCited times in Scopus
Share
អរូបី
Micropropagation has proven to be a reliable method to mass produce certain crops. This method also has been applied in macroalgae to produce clones for seaweed farming. Protocols for callus production and shoot regeneration from protoplasts have been established for some seaweed species like Kappaphycus alvarezii. Cells and larger tissues, whether in solid or suspension medium, have been used to propagate clones which were later tested for suitability for farming. Although clonal production was successful, the long duration of culture in vitro limits the production process making the growing of Kappaphycus in vitro an expensive technique to produce clones. In this study, K. alvarezii was grown in vitro to develop a more efficient protocol for the production of clones. Small sections of Kappaphycus were grown in suspension for 1 month under the same temperature, light, and salinity. The type of media, source of explants, length of explants, and stocking density that resulted in the highest growth rate and survival rate were determined. Growth rate of K. alvarezii is significantly higher in media with inorganic nitrogen added than in Grund medium or Ascophyllum nodosum medium only. The appearance of shoot primordia as early as 5 days was observed in media with higher nitrogen concentration. Growth rates of explants approximately 3 and 5 mm are significantly higher than 10 mm sections. Shoots develop significantly faster in explants from tips than sections from older branches. Growth rate of K. alvarezii grown at 0.5, 0.75, 1, 1.25 s 10 mL−1 of medium is not significantly different. This protocol could significantly reduce the (1) time of culture and (2) cost of plantlets production by not using plant growth regulators and formulated media in vitro. Nursery reared plantlets/propagules for farming would be affordable to the stakeholders for sustainability of seaweed production.
Suggested Citation
Luhan, M. R. J., & Mateo, J. P. (2017). Clonal production of Kappaphycus alvarezii (Doty) Doty in vitro. Journal of Applied Phycology , 29, 2339-2344. https://doi.org/10.1007/s10811-017-1105-7
ប្រធានបទ
Taxonomic term
បណ្តុំបណ្តុំ
- AQD Journal Articles [1240]
Related items
Showing items related by title, author, creator and subject.
-
Biyah diin in pagparagan sin pagtanuman agal agal iban pag'ayad ha pag' urulan sin kabuhi: Binhi atawa tanumun agal agal, manga mangangaun iban kumakangii' iban sakit, manga anib atawa makamula' iban biyah diin in pagpahilunnah sin anib atawa piligru ha pagtanum agal agal
Mateo, Jonalyn; Faisan, Jr., Joseph; Sibonga, Rema; Suyo, Jee Grace; Luhan, Maria Rovilla; Ferriols, Victor Marco Emmanuel; Hurtado, Anicia (United Kingdom Research and Innovation-Global Challenge Research Fund (UKRI-GCRF), 2021-03)In buwk-buwk pahati ini nahinang iban kiyasulat paglabay upat tahun ha pagsaliksik ha pag’usaha agal agal ha Pilipinas hinang sin tumpukan sin GlobalSeaweedSTAR Philippine ha bitsara kahatihan sin kamatauran. Makatagama ... -
Pangangasiwa sa taniman at mga hakbang pangbayo-sekyuridad para sa Eucheumatoids: Mga kultibar, peste at sakit, mga risgo o peligro at mga paraan sa pangasiwaan nito
Mateo, Jonalyn; Faisan, Jr., Joseph; Sibonga, Rema; Suyo, Jee Grace; Luhan, Maria Rovilla; Ferriols, Victor Marco Emmanuel; Hurtado, Anicia (United Kingdom Research and Innovation-Global Challenge Research Fund (UKRI-GCRF), 2021-03)Ang polyeto na ito ay inihanda at isinulat ng GlobalSeaweedSTAR Philippine Team sa payak na lengwahe matapos ang apat na taong pananaliksik sa industriya ng pag-aalaga ng mga halamang-dagat sa Pilipinas. Nais nito na ... -
Pagdumala sa uma sa guso ug mga lakang para sa bayo-sekuridad: Semilya, mga peste ug sakit-sakit, mga risgo ug pagdumala niini
Mateo, Jonalyn; Faisan, Jr., Joseph; Sibonga, Rema; Suyo, Jee Grace; Luhan, Maria Rovilla; Ferriols, Victor Marco Emmanuel; Hurtado, Anicia (United Kingdom Research and Innovation-Global Challenge Research Fund (UKRI-GCRF), 2021-03)Gisulat kining brosyur matapos ang upat ka tuig nga panukiduki sa ‘GlobalSeaweedSTAR Philippine Team’ sa industriya sa guso sa Pilipinas gamit ang simple nga sinultian nga daling masabtan sa mga layko. Kini magahatag ug ...