Effects of arachidonic acid supplementation on larval and survival and reproductive performance in rabbitfish, Siganus guttatus
Fry of tropical marine fish needed for aquaculture still comes mostly from the wild. Thus, fry availability is a major constraint in the development and extension of aquaculture, especially in rural areas of developing regions. Although the mission of hatcheries is to provide a stable fry production and supply for farmers, fry production remains variable due to poor fecundity and low survival. For the last four years (2002-2005), SEAFDEC/AQD and JIRCAS have conducted the collaborative project that was aimed at developing advanced diets for improving egg production/quality (2002-2005) and larvae/fry quality (2004-2005) through dietary manipulation. Larval rearing tests: In 2005, larval rearing tests (4 trials with rotifers) were conducted to investigate the effects of enriched-live food (4treatments: low (CS) and high (DHAPS) HUFA with or without arachidonic acid supplementation) on survival and growth in rabbitfish Sigunus guttatus fry. Fry fed the rotifers enriched with a combination of DHAPS+5% ArA showed the best survival (44.4±4.5% for D17 fry in the 4th trial).Growth was not different among the treatments (CS, CS+5% ArA, DHAPS, DHPS+5% ArA). Broodstock tests: From March, 2005 to January,2006, a feeding test has been conducted to investigate the effects of dietary ArA supplementation (0% for diet 1, 0.3% for diet 2 and 0.6% for diet 3) on egg production and quality of wild-caught and hatchery-bled rabbitfish broodstock. The broodstock spawned 13 times for diet1 (six pairs), 14 times for diet 2 (five pairs) and 17 times for diet 3 (six pairs) during the period of May 2005 to January, 2006. The total numbers ofhatched-larvae were 3,818 x 103 for diet 1, 4,391 x 103 for diet 2 and 4,597 x 103 for diet 3. The % of normal larvae did not differ among the dietary treatments. Considering together with the results of mangrove red snapper (2003) and rabbitfish (2004), the optimum level of ArA incorporation appears to be between 0.5% and 0.7%. Judging from the results of fatty acid analysis, DHA and arachidonic acid should be supplemented to diets at the same time as to make DHA/arachidonic acid ratio appropriate. Thus, the present study clearly shows that dietary arachidonic acid supplementation is very promising for the development of fry production technologies in tropical areas.
Chavez, D. R., Ogata, H. Y., Garibay, E. S., Sollesta, H. T., Tibubos, K. R., Furuita, H., & Suloma, A. (2007). Effects of arachidonic acid supplementation on larval and survival and reproductive performance in rabbitfish, Siganus guttatus. In K. Nakamura (Ed.), Sustainable Production Systems of Aquatic Animals in Brackish Mangrove Areas (JIRCAS Working Report No. 56) (pp. 113–120). Tsukuba, Ibaraki, Japan: Japan International Research Center for Agricultural Sciences.
- AQD 
Analysis of additives: Determination of sorbic acid, benzoic acid and dehydroacetic acid by steam distillation and UV spectrophotometric method Yamagata, Makoto (Marine Fisheries Research Department, Southeast Asian Fisheries Development Center, 1992)
Arachidonic acid is a major fatty acid in gonads of coral reef fishes and improves larval survival of rabbitfish Sigunus gutattus Suloma, Ashraf; Chavez, Denny R.; Garibay, Esteban S.; Furuita, Hirofumi; Ogata, Hiroshi Y. (Nova Science Publishers, 2016)The supply of wild fry of coral reef fishes for aquaculture has resulted in the deterioration of their natural stock status, causing public concern. Through a series of studies on the establishment of artificial-fry ...
Benitez, Lita V. (Asian Fisheries Society, 1989)The amino acid profile is an important parameter in the evaluation of protein quality and in requirement studies. Amino acid profiles are usually determined in 2 steps: hydrolysis of the protein to constituent amino acids ...