Evaluation of free essential amino acid in muscle of Nile tilapia (Oreochromis niloticus), as a basis of amino acid requirement for growth.
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The free essential amino acid levels in muscle of young Nile tilapia (Oreochromis niloticus) after feeding graded amounts of a corresponding test amino acid were measured in 9 separate experiments to determine if free amino acids indicate requirements for growth. Data were analyzed by the broken line regression method. Breakpoints for free threonine and free isoleucine were near the threonine and isoleucine requirements for growth. Breakpoints for free lysine, free histidine, and free valine were much higher than the lysine, histidine, and valine requirements for growth, respectively. Levels of other free essential amino acids in muscle behaved erratically or increased linearly as the dietary test amino acid increased. Amino acid requirements as estimated by levels of free essential amino acids in muscle did not consistently confirm amino acid requirements for growth of Nile tilapia.
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Santiago, Corazon B.; Lovell, Richard T. (American Society for Nutrition, 1988)A series of feeding experiments was conducted in aquaria to determine the quantitative requirements of the 10 essential amino acids for growth of young Nile tilapia (Oreochromis niloticus). The test diets contained casein ...
Preliminary studies in some aspects of amino acid biosynthesis in juveniles of Penaeus monodon Fabricius: I. Incorporation of 14C from (U-14C) acetate into amino acids to precipitable proteins Coloso, Relicardo M.; Cruz, Lourdes J. (Philippine Biochemical Society, 1980)Ion-exchange chromatography in conjunction with radioisotope labelling techniques were used to investigate the incorporation of <sup>14</sup>C from (U-<sup>14</sup>C) - acetate into amino acids of precipitable proteins in ...
Benitez, Lita V. (Asian Fisheries Society, 1989)The amino acid profile is an important parameter in the evaluation of protein quality and in requirement studies. Amino acid profiles are usually determined in 2 steps: hydrolysis of the protein to constituent amino acids ...