Milt-egg ratio in artificial fertilization of the Asian freshwater catfish, Clarias macrocephalus, injected salmon gonadotropin-releasing hormone analogue and domperidone

Abstract

The author deals with the catfish Clarias macrocephalus which are artificially fertilized by inducing females to spawn using various hormones. This paper investigates the effect of Ovaprim on milt production and fertilizing ability of Clarias macrocephalus and determines the optimal milt-egg ratio required for artificial fertilization. Materials and methods used are: fish collection and handling, hormone administration, preparation of testicular homogenate, measurement of sperm density, dry fertilization, statistical analysis.