Artificial fertilization of eggs and early development of the milkfish Chanos chanos (Forskal)
Hydrated eggs obtained from a female milkfish were artificially fertilized with the milt collected from a male injected with acetone-dried pituitaries of salmon. The fertilized eggs (1.1 to 1.25 mm in diameter) developed normally in seawater in basins and Petri dishes at a salinity of 30-34 ppt and successfully hatched in 25 to 28½ hours at a temperature of 26.4-29.9°C. The yolk was completely absorbed in about 2½ days and at this period many postlarvae died. A few larvae were reared up to 5 days but all died within 6 days. Effects of feeding the postlarvae from the third day with freshly hatched trochophore larvae of oysters obtained from eggs artificially fertilized in the laboratory could not be ascertained.
Contribution No. 12 of the Aquaculture Department, Southeast Asian Fisheries Development Center, P.O. Box 256, Iloilo City 5901, Philippines.
Chaudhuri, H., Juario, J. V., Primavera, J. H., Mateo, R., Samson, R., Cruz, E., … Canto, J. J. (1977). Artificial fertilization of eggs and early development of the milkfish Chanos chanos (Forskal). In: Induced spawning, artificial fertilization of eggs and larval rearing of the milkfish Chanos chanos (Forskal) in the Philippines (Technical Report No. 3) (pp. 21-38). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) Requintina, Pura D.; Engle, L. M.; Benitez, Lita V. (University of the Philippines at Los Baños, 1981)Polyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were ...
Benitez, Lita V. (Philippine Council for Aquatic and Marine Research and Development, 1989)This paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and ...
Villaluz, Antonio C. (The Oceanic Institute, 1986)A review is made of methods and practices of collection, storage, transport and acclimation of milkfish (Chanos chanos ) fry and fingerlings in various countries. Factors affecting catch and survival are examined.