dc.contributor.author | Hara, Shiro | |
dc.contributor.author | Canto, Jose T., Jr. | |
dc.contributor.author | Almendras, Jesus M. E. | |
dc.date.accessioned | 2012-08-24T09:15:56Z | |
dc.date.available | 2012-08-24T09:15:56Z | |
dc.date.issued | 1982 | |
dc.identifier.citation | Hara, S., Canto, J., Jr.T., & Almendras, J.M.E. (1982). A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28(3-4), 339-346. doi:10.1016/0044-8486(82)90076-X | en |
dc.identifier.issn | 0044-8486 | |
dc.identifier.uri | http://hdl.handle.net/10862/1041 | |
dc.description.abstract | Various extenders, containing potassium chloride, sodium chloride, glucose, sodium citrate, Ringer's solution, cow serum and milkfish serum were used to preserve milkfish (Chanos chanos) sperm at near-zero temperatures (0-4°C) and in liquid nitrogen (?196°C). Milkfish serum was a superior extender in both cases. After 5 days, comparatively good motility (> 30%) and fertilizing capacity (6.7-18.9%) were observed in the near-zero liquid samples, while in other extenders, sperm ceased to show motility after 2 days. The fertilization success of 4-5 days cryopreserved sperm averaged 67.5% (n = 2) with milkfish serum, 60.5% (n = 2) with 400 mM glucose, 58.0% (n = 2) with 150 mM sodium chloride, 41.2% (n = 1) with Ringer's solution and 31.9% (n = 2) with cow serum. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Chanos chanos | en |
dc.subject | Freezing storage | en |
dc.subject | milkfish | en |
dc.title | A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation | en |
dc.type | Article | en |
dc.citation.volume | 28 | |
dc.citation.issue | 3-4 | |
dc.citation.spage | 339 | |
dc.citation.epage | 346 | |
dc.citation.journalTitle | Aquaculture | en |
dc.subject.asfa | sperm | en |
dc.subject.asfa | preservatives | en |
dc.subject.asfa | aquaculture techniques | en |
dc.subject.asfa | fertilization | en |
dc.identifier.doi | 10.1016/0044-8486(82)90076-X | |
dc.subject.scientificName | Chanos chanos | |