Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration
Quan điểm/ Open
Request this document
Ngày
2019-03Tác giả
Page views
430metadata
Hiển thị bản ghi đầy đủ mặt hàngCited times in Scopus
Share
trừu tượng
The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control). Sustained treatment with rggFSH (20 to 38 weeks at 200 μg/kg BW) resulted in significant reduction in gonad size, degeneration of oocytes and proliferation of spermatogonial cells, indicative of female to male sex change. Gene expression analysis showed that, while initiating female to male sex change, the rggFSH significantly suppressed the steroidogenic genes cyp11b, cyp19a1a and foxl2 which restrained the endogenous production of sex steroid hormones thus prevented the differentiation of spermatogonial cells. Expression profile of sex markers dmrt1, amh, figla and bmp15 suggests that the observed sex change was restricted at the initiation stage. Based on these results, we propose that the process of female to male sex change in the protogynous grouper is initiated by FSH, rather than sex steroids and likely involves steroid-independent pathway. The cortical alveolar stage in oocyte development is the critical point after which FSH-induced sex change is possible in grouper.
Suggested Citation
Palma, P., Nocillado, J., Superio, J., Ayson, E. G. d. J., Ayson, F., Bar, I., & Elizur, A. (2019). Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration. Biology of Reproduction , 100(3), 798-809. https://doi.org/10.1093/biolre/ioy228
Chủ thể
Taxonomic term
Bộ sưu tập
- AQD Journal Articles [1223]
Related items
Showing items related by title, author, creator and subject.
-
Effects of different fat sources on the egg quality of grouper, Epinephelus suillus
Quinitio, G. F.; Coloso, R. M.; Duller, A.; Reyes, D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996)The effect of different fat sources on the egg quality of grouper, Epinephelus suillus eggs was evaluated. Fish in three tanks, each containing 3 females and 1 male, were fed various types of feeds namely: trash fish ... -
A review of grouper (Epinephelus suillus) fry production research in Malaysia
Ali, Hussin Mat (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996)Research on grouper (Epinephelus suillus) fry production in captivity has been carried out in Malaysia since 1986 at Tanjung Demong Marine Finfish Production and Research Centre (TDMFPRC) but the breakthrough was only ... -
Research on marine and freshwater fishes
Emata, Arnil C. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1995)Most of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for ...