SEAFDECINSTITUTIONAL REPOSITORY
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • English 
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • Login
View Item 
  •   SEAFDEC Institutional Repository (SIR)
  • 01 SEAFDEC Publications
  • 11 Conference Proceedings
  • SEAFDEC/AQD
  • Aquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia (AEPRS)
  • View Item
  •   SEAFDEC Institutional Repository (SIR)
  • 01 SEAFDEC Publications
  • 11 Conference Proceedings
  • SEAFDEC/AQD
  • Aquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia (AEPRS)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Emergency response to emerging diseases: TiLV in tilapia

Thumbnail
View/Open
SenapinS2019.pdf (85.07Kb)
Date
2019
Author
Senapin, Saengchan
Page views
168
Metadata
Show full item record

Share 
 
Abstract
Tilapia lake virus (TiLV) is a novel RNA virus resembling Orthomyxovirus. It has been recently re-classified to Tilapia tilapinevirus species, under Tilapinevirus genus, Amnoonviridae family (ICTV, 2018). Since the first discovery in Israel in 2014, so far TiLV has been reported from 14 countries in three continents (Asia, Africa, and South America). Thailand is one of the affected countries that reported emergence of this virus in 2017. Initially, we employed nested RT-PCR primer sequences previously published for TiLV diagnosis. However, the resulting amplification of nonspecific fish genes led us to modify the nested RT-PCR protocols into a semi-nested RT-PCR by omitting a non-specific primer to avoid false positive results. Subsequently, our molecular work together with histopathology and sequence analysis confirmed the presence of TiLV infection in Thailand. Prior to the publication of our manuscript, we informed the Thai Department of Fisheries of our discovery of TiLV in Thailand. Our publication was preceded by a brief article at the website of the Network of Aquaculture Centers in Asia-Pacific in which we warned of the spread of TiLV and offered free use of a newly improved, semi-nested RT-PCR method and positive control plasmid for detection of TiLV. To date, we have provided positive controls in response to 44 requests from 24 countries who have expressed their appreciation for our attempt to help in emergent controlling the spread of this fish pathogen. Our current study focuses on genetic diversity of TiLV and development of detection method that covers all genetic variants.
URI
http://hdl.handle.net/10862/3468
Suggested Citation
Senapin, S. (2019). Emergency response to emerging diseases: TiLV in tilapia. In E. A. Tendencia, L. D. de la Peña, & J. M. V. de la Cruz (Eds.), Aquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia: Proceedings of Asean Regional Technical Consultation, 20-22 August 2018, Centara Grand Central Ladprao, Bangkok, Thailand (pp. 81-83). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Subject
Disease control; Disease detection; Disease resistance; Disease transmission; Epidemiology; Husbandry diseases; Aquaculture regulations; Quarantine regulations; fish culture ASFA; fish diseases ASFA; freshwater aquaculture ASFA; Freshwater fish; Viral diseases; Infectious diseases; Virology; Viruses; Genetics; Emergencies; Freshwater; Tilapinevirus; Hepatopancreas; Polymerase chain reaction; Fisheries; Genes; Fish; Nucleic acids; Lakes; Pathogens; Protocols; RNA; aquaculture ASFA; Histopathology; Detection; Nucleotide sequence; Plasmids; DNA; Inland waters; Freshwater fish; Genetic diversity; Countries; Vibrio parahaemolyticus; Vibrio campbellii; Vibrio harveyi; Vibrio owensii; Amnoonviridae; Asia; Thailand
Collections
  • Aquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia (AEPRS) [20]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Aquatic emergency preparedness and response system in Viet Nam 

    Bui, Viet-Hang T.; Nguyen, Viet-Nga T.; Nguyen, Lan-Huong T.; Nguyen, Hien T.; Pham, Quan H.; Vo, Chuong D.; Nguyen, Tien N. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2019)
    Viet Nam is one of the top worldwide producers of aquaculture products which accounts for about 22 percent of total agricultural GDP of Viet Nam. Recently, diseases have become the biggest challenge for global aquaculture ...
  • Thumbnail

    Philippines: Aquatic emergency preparedness and response systems for transboundary diseases 

    Somga, Sonia S.; Somga, Joselito R.; Quiatchon, Gladys M.; Regidor, Simeona E. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2019)
    The Bureau of Fisheries and Aquatic Resources (BFAR) of the Department of Agriculture as the Competent Authority, develops and implements rules and regulations on aquatic animal health for the Philippines. It establishes ...
  • Thumbnail

    Effect of shrimp biomass and feeding on the anti-Vibrio harveyi activity of Tilapia sp. in a simulated shrimp–tilapia polyculture system 

    Tendencia, Eleonor; dela Peña, Milagros R.; Choresca Jr., Casiano H. (Elsevier, 2006)
    The efficiency of Tilapia hornorum to control luminous bacteria in a simulated shrimp farm environment has been reported. However, the effects of different factors such as feed input and the shrimp biomass were not taken ...

© SEAFDEC 2022
Contact Us | Send Feedback
 

 

Browse

All of SIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics
Related Links
SEAFDEC/TD IRSEAFDEC/AQD IRSEAFDEC/MFRDMD IRSEAFDEC/IFRDMD IR

© SEAFDEC 2022
Contact Us | Send Feedback