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dc.contributor.authorSenapin, Saengchan
dc.contributor.editorTendencia, Eleonor A.
dc.contributor.editorde la Peña, Leobert D.
dc.contributor.editorde la Cruz, Joesyl Marie V.
dc.date.accessioned2019-07-23T08:09:34Z
dc.date.available2019-07-23T08:09:34Z
dc.date.issued2019
dc.identifier.citationSenapin, S. (2019). Emergency response to emerging diseases: TiLV in tilapia. In E. A. Tendencia, L. D. de la Peña, & J. M. V. de la Cruz (Eds.), Aquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia: Proceedings of Asean Regional Technical Consultation, 20-22 August 2018, Centara Grand Central Ladprao, Bangkok, Thailand (pp. 81-83). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9789719931089
dc.identifier.urihttp://hdl.handle.net/10862/3468
dc.description.abstractTilapia lake virus (TiLV) is a novel RNA virus resembling Orthomyxovirus. It has been recently re-classified to Tilapia tilapinevirus species, under Tilapinevirus genus, Amnoonviridae family (ICTV, 2018). Since the first discovery in Israel in 2014, so far TiLV has been reported from 14 countries in three continents (Asia, Africa, and South America). Thailand is one of the affected countries that reported emergence of this virus in 2017. Initially, we employed nested RT-PCR primer sequences previously published for TiLV diagnosis. However, the resulting amplification of nonspecific fish genes led us to modify the nested RT-PCR protocols into a semi-nested RT-PCR by omitting a non-specific primer to avoid false positive results. Subsequently, our molecular work together with histopathology and sequence analysis confirmed the presence of TiLV infection in Thailand. Prior to the publication of our manuscript, we informed the Thai Department of Fisheries of our discovery of TiLV in Thailand. Our publication was preceded by a brief article at the website of the Network of Aquaculture Centers in Asia-Pacific in which we warned of the spread of TiLV and offered free use of a newly improved, semi-nested RT-PCR method and positive control plasmid for detection of TiLV. To date, we have provided positive controls in response to 44 requests from 24 countries who have expressed their appreciation for our attempt to help in emergent controlling the spread of this fish pathogen. Our current study focuses on genetic diversity of TiLV and development of detection method that covers all genetic variants.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectVibrio parahaemolyticus
dc.subjectVibrio campbellii
dc.subjectVibrio harveyi
dc.subjectVibrio owensii
dc.subjectAmnoonviridae
dc.subjectAsiaen
dc.subjectThailanden
dc.titleEmergency response to emerging diseases: TiLV in tilapiaen
dc.typeConference paperen
dc.citation.spage81
dc.citation.epage83
dc.subject.asfadisease controlen
dc.subject.asfaDisease detectionen
dc.subject.asfadisease resistanceen
dc.subject.asfadisease transmissionen
dc.subject.asfaepidemiologyen
dc.subject.asfahusbandry diseasesen
dc.subject.asfaaquaculture regulationsen
dc.subject.asfaquarantine regulationsen
dc.subject.asfafish cultureen
dc.subject.asfafish diseasesen
dc.subject.asfafreshwater aquacultureen
dc.subject.asfaFreshwater fishen
dc.subject.asfaviral diseasesen
dc.subject.asfainfectious diseasesen
dc.subject.asfavirologyen
dc.subject.asfavirusesen
dc.subject.asfageneticsen
dc.subject.asfaemergenciesen
dc.subject.asfafreshwateren
dc.subject.asfaTilapinevirusen
dc.subject.asfahepatopancreasen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfafisheriesen
dc.subject.asfagenesen
dc.subject.asfafishen
dc.subject.asfanucleic acidsen
dc.subject.asfalakesen
dc.subject.asfapathogensen
dc.subject.asfaprotocolsen
dc.subject.asfaRNAen
dc.subject.asfaaquacultureen
dc.subject.asfahistopathologyen
dc.subject.asfadetectionen
dc.subject.asfanucleotide sequenceen
dc.subject.asfaplasmidsen
dc.subject.asfaDNAen
dc.subject.asfainland watersen
dc.subject.asfaGenetic diversityen
dc.subject.asfacountriesen
dc.citation.conferenceTitleAquatic Emergency Preparedness and Response Systems for Effective Management of Transboundary Disease Outbreaks in Southeast Asia: Proceedings of Asean Regional Technical Consultation, 20-22 August 2018, Centara Grand Central Ladprao, Bangkok, Thailanden


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