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Bacterial microbiota of hatchery-reared freshwater prawn Macrobrachium rosenbergii (De Man, 1879) and their pathogenicity

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Date
2022
Author
Monghit-Camarin, Ma-ann
Cruz-Lacierda, Erlinda R.
Pakingking, Rolando
Cuvin-Aralar, Maria Lourdes
Traifalgar, Rex Ferdinand
Añasco, Nathaniel C.
Austin, Frank William
Lawrence, Mark Lee
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Abstract
Quantitative and qualitative analyses of bacterial microbiota associated with hatchery of Macrobrachium rosenbergii (de Man, 1879) were conducted over three larval cycles, along with important water quality parameters. Physicochemical parameters (temperature, DO, salinity, pH, and total ammonia) of culture water were within the optimum level required for the hatchery phase of M. rosenbergii. Significant differences in aerobic plate count (APC) among larval stages were observed. Ten genera and 17 species were identified with the predominance of Gram-negative bacteria constituting 63 % of all isolates (n = 706). A total of 17 dominant bacterial isolates randomly selected from freshwater prawn larvae were screened for in vitro hemolysin activity using blood agar plate (BAP) medium with 5% sheep blood. Pathogenicity of the isolated bacterial strains was assessed through in vivo infection bioassay. Out of the 17 isolates tested, six isolates showed α – β zone of hemolysis on the BAP, i.e., Aeromonas caviae, Bacillus cereus, Plesiomonas shigelloides, Staphylococcus warneri, Vibrio cholerae non-01, and Vibrio furnissii, were used in the infection bioassay. The current data indicate that the six bacterial isolates tested, particularly B. cereus and V. cholerae non-01, are opportunistic pathogens capable of inducing disease epizootics, particularly in freshwater prawn hatcheries with suboptimal rearing water conditions. Thus, control measures to reduce the influx of pathogenic microbes in the system by maintaining good water quality and good farm management practices through disinfection of culture facilities, rearing water, and Artemia cysts, the practice of good hygiene of personnel, regular water exchange, and feed regulation, among others, and cautionary use of antibiotics, can be adopted.
Keywords
Quantitative analysis species composition qualitative normal flora pathogen
URI
http://hdl.handle.net/10862/6418
Suggested Citation
Monghit-Camarin, M., Cruz-Lacierda, E., Pakingking, Jr., R. V., Cuvin-Aralar, M. L., Traifalgar, R. F. M., Añasco, N. C., Austin, F. W., & Lawrence, M. L. (2022). Bacterial microbiota of hatchery-reared freshwater prawn Macrobrachium rosenbergii (De Man, 1879) and their pathogenicity. In A. J. G. Ferrer (Ed.), Proceeding of Joint International Conference of the Sixth International Conference on Fisheries and Aquatic Sciences (ICFAS 6) and Asian Fisheries Social Science Research Network Forum One (AFSSRN F1), 24-26 November 2021, via Zoom, Miagao, Iloilo, Philippines (pp. 209–215). University of the Philippines Visayas.
Subject
Bacteria ASFA; shrimp fisheries ASFA; pathogenic bacteria ASFA; pathogens ASFA; culture tanks ASFA; water temperature ASFA; dissolved oxygen ASFA; pH ASFA; salinity ASFA; ammonia ASFA; shrimp culture ASFA; water quality ASFA; flora ASFA; prawn culture ASFA; aquaculture ASFA; bacteriology ASFA; Macrobrachium rosenbergii AGROVOC; Bacillus cereus AGROVOC; Plesiomonas shigelloides AGROVOC; Vibrio cholerae AGROVOC; Artemia AGROVOC
Taxonomic term
Macrobrachium rosenbergii GBIF; Aeromonas caviae GBIF; Bacillus cereus GBIF; Plesiomonas shigelloides GBIF; Staphylococcus warneri GBIF; Vibrio cholerae GBIF; Vibrio furnissii GBIF; Artemia GBIF
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