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Establishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus)

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Associated URL
www.asianfisheriessociety.org
Date
1999
Author
Lio-Po, Gilda D.
Traxler, G. S.
Albright, L. J.
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103
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Abstract
Primary cell cultures from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus) were prepared from whole fry and fingerling organ tissues of the brain, fins, gonad, heart, kidney, liver, skin and spleen. Four methods were tried: method A, wherein explants were placed onto the surface of 25-cm2 Primaria flasks (Falcon), allowed to attach for an hour before addition of Leibovitz medium (L-15) supplemented with 15% fetal bovine serum (FBS)(L15-15); Method B, wherein explants were inoculated into 25-cm2 Primaria flasks (Falcon) already containing L15-15; Method C, which required forcing minced organ sections through a stainless steel sieve with the aid of a syringe plunger into a petri dish containing L15-15 medium; and Method D, wherein immersed sections of minced tissues to 0.5% trypsin-EDTA were slowly agitated using a magnetic stirrer for one hour at 25°C. Method B was most effective in the establishment of cell cultures from both fish species. Passage numbers of the cells are to date catfish gonad (CFG) P-56, catfish heart (CFH) P-51, catfish kidney (CFK) P-7, catfish liver (CFL) P-8, catfish spleen (CFS) P-54, snakehead gonad (SHG) P-26, snakehead heart (SHH) P-22, snakehead kidney (SHK) P-19, snakehead liver (SHL) P-49 and snakehead spleen (SHS) P-76. Attempts to derive primary cell cultures from organ tissues of the brain, fins, skin and whole fry were unsuccessful. Established cells were fibroblastic. The cells grew rapidly and became confluent 24 h after seeding at 20 and 25°C. Both SHS and CFS were susceptible to a virus isolated from EUS-affected fish in the Philippines. The cells were best maintained at 20°C and stored in liquid nitrogen or -70°C.
URI
http://hdl.handle.net/10862/1839
Suggested Citation
Lio-Po, G. D., Traxler, G. S., & Albright, L. J. (1999). Establishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus). Asian Fisheries Science, 12(4), 343-349.
Subject
cell culture ASFA; disease resistance ASFA; fish diseases ASFA; tissue culture ASFA; Clarias batrachus AGROVOC; Channa striatus AGROVOC
Taxonomic term
Ophicephalus striatus GBIF; Channa striata GBIF
Collections
  • AQD Journal Articles [1175]

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    Characterization of a virus obtained from snakeheads Ophicephalus striatus with epizootic ulcerative syndrome (EUS) in the Philippines 

    Lio-Po, Gilda D.; Traxler, Garth S.; Albright, Lawrence J.; Leaño, Eduardo M. (Inter Research, 2000)
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    Lio-Po, Gilda D.; Albright, Lawrence J.; Traxler, Garth S.; Leaño, Eduardo M. (Inter Research, 2003)
    Natural transmission of the epizootic ulcerative syndrome (EUS) was conducted on naïve snakeheads Ophicephalus striatus (also known as Channa striata) kept (A) in aquifer water, (B) in lakewater, (C) cohabiting with EUS ...
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    Experimental transmission of epizootic ulcerative syndrome (EUS) in snakehead, Ophicephalus striatus 

    Cruz-Lacierda, Erlinda R.; Shariff, Mohamed (Fish Health Section, Asian Fisheries Society, 1995)
    Two separate experiments on transmission of epizootic ulcerative syndrome (EUS) to naive snakehead (Ophicephalus striatus) by cohabitation with EUS-positive snakehead in EUS-enzootic environment and exposure to EUS-enzootic ...

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