SEAFDECINSTITUTIONAL REPOSITORY
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • English 
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • Login
View Item 
  •   SEAFDEC Institutional Repository (SIR)
  • 03 SEAFDEC External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • AQD Journal Articles
  • View Item
  •   SEAFDEC Institutional Repository (SIR)
  • 03 SEAFDEC External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • AQD Journal Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Establishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus)

Thumbnail
Associated URL
www.asianfisheriessociety.org
Date
1999
Author
Lio-Po, Gilda D.
Traxler, G. S.
Albright, L. J.
Page views
61
Metadata
Show full item record

Share 
 
Abstract
Primary cell cultures from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus) were prepared from whole fry and fingerling organ tissues of the brain, fins, gonad, heart, kidney, liver, skin and spleen. Four methods were tried: method A, wherein explants were placed onto the surface of 25-cm2 Primaria flasks (Falcon), allowed to attach for an hour before addition of Leibovitz medium (L-15) supplemented with 15% fetal bovine serum (FBS)(L15-15); Method B, wherein explants were inoculated into 25-cm2 Primaria flasks (Falcon) already containing L15-15; Method C, which required forcing minced organ sections through a stainless steel sieve with the aid of a syringe plunger into a petri dish containing L15-15 medium; and Method D, wherein immersed sections of minced tissues to 0.5% trypsin-EDTA were slowly agitated using a magnetic stirrer for one hour at 25°C. Method B was most effective in the establishment of cell cultures from both fish species. Passage numbers of the cells are to date catfish gonad (CFG) P-56, catfish heart (CFH) P-51, catfish kidney (CFK) P-7, catfish liver (CFL) P-8, catfish spleen (CFS) P-54, snakehead gonad (SHG) P-26, snakehead heart (SHH) P-22, snakehead kidney (SHK) P-19, snakehead liver (SHL) P-49 and snakehead spleen (SHS) P-76. Attempts to derive primary cell cultures from organ tissues of the brain, fins, skin and whole fry were unsuccessful. Established cells were fibroblastic. The cells grew rapidly and became confluent 24 h after seeding at 20 and 25°C. Both SHS and CFS were susceptible to a virus isolated from EUS-affected fish in the Philippines. The cells were best maintained at 20°C and stored in liquid nitrogen or -70°C.
URI
http://hdl.handle.net/10862/1839
Suggested Citation
Lio-Po, G. D., Traxler, G. S., & Albright, L. J. (1999). Establishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus). Asian Fisheries Science, 12(4), 343-349.
Subject
cell culture ASFA; disease resistance ASFA; fish diseases ASFA; tissue culture ASFA; Clarias batrachus AGROVOC; Channa striatus AGROVOC
Taxonomic term
Ophicephalus striatus GBIF; Channa striata GBIF
Collections
  • AQD Journal Articles [1155]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Parasitic crustaceans in fishes from some Philippine Lakes 

    Lopez, Nellie C. (Aquaculture Department, Southeast Asian Fisheries Development Center; Philippine Council for Aquatic and Marine Research and Development (PCAMRD), Department of Science and Technology; Bureau of Fisheries and Aquatic Resources, 2001)
    Parasitic crustaceans are among the most harmful parasites of fishes. Certain species cause disease outbreaks and mortalities in aquaculture, facilities, and sometimes in natural systems, resulting in serious economic ...
  • Thumbnail

    Experiments on virulence dose and portals of entry for Aeromonas hydrophila in walking catfish 

    Lio-Po, Gilda D.; Albright, Lawrence J.; Leaño, Eduardo M. (American Fisheries Society, 1996)
    Aeromonas hydrophila, isolated from chevron snakehead Ophicephalus (=Channa) striatus affected with epizootic ulcerative syndrome (EUS), was injected intramuscularly into healthy walking catfish Clarias batrachus at varying ...
  • Thumbnail

    Restoration and Enhancement of Fisheries in Philippine Lakes and Reservoirs 

    Palma, Adelaida L. (Training Department, Southeast Asian Fisheries Development Center, 2016)
    Inland water resources of the Philippines host some 348 freshwater fishes, of which 16% are endemic and 56% indigenous (FishBase 2013). Although production from inland capture fisheries accounts for only 7.8% of the total ...

© SEAFDEC 2022
Contact Us | Send Feedback
 

 

Browse

All of SIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics
Related Links
SEAFDEC/TD IRSEAFDEC/AQD IRSEAFDEC/MFRDMD IRSEAFDEC/IFRDMD IR

© SEAFDEC 2022
Contact Us | Send Feedback
 

 

EXTERNAL LINKS DISCLAIMER

This link is being provided as a convenience and for informational purposes only. SEAFDEC bears no responsibility for the accuracy, legality or content of the external site or for that of subsequent links. Contact the external site for answers to questions regarding its content.

If you come across any external links that don't work, we would be grateful if you could report them to the repository administrators.

Click DOWNLOAD to open/view the file.

Download