Milkfish (Chanos chanos) growth hormone cDNA cloning and mRNA expression in embryos and early larval stages.

View/ Open
Request this document
Date
2002Author
Page views
146Metadata
Show full item recordCited times in Scopus
Share
Abstract
In an attempt to understand growth regulation in milkfish, the milkfish growth hormone (GH) and its cDNA were characterized and the expression of GH mRNA in embryos and larvae was examined by RT-PCR. The milkfish GH was purified from an alkaline extract of the pituitary by reverse-phase high-performance liquid chromatography and detected as an immuno-positive protein with anti-salmon GH serum. The complete sequence of milkfish pre-GH was determined by cDNA cloning and nucleotide sequencing. On the basis of the N-terminal amino acid analysis of the native protein, the pre-GH was found to consist of a signal peptide of 22 amino acids and a mature protein of 188 amino acids. Milkfish GH shows higher amino acid sequence identity with GHs of carps (91–94%) and salmonids (70%) than with GHs of more advanced teleosts (<60%) in good accordance with its taxonomic position in teleosts. It has five half Cys residues, four of which are at positions homologous with those of other known GHs and the extra Cys with those of carp GHs. The molecular weight of milkfish GH was estimated to be 22 kDa, which is comparable to the theoretical value. This suggests that milkfish GH is a simple protein, although it has two potential N-glycosylation sites. Semiquantitative RT-PCR showed that GH mRNA expression was relatively weak in embryos and newly hatched larvae but was already strong in 2-day old and older larvae.
Suggested Citation
de Jesus, E. G. T., Ayson, F. G., Amemiya, Y., Moriyama, S., Hyodo, S., Hirano, T., & Kawauchi, H. (2002). Milkfish (Chanos chanos) growth hormone cDNA cloning and mRNA expression in embryos and early larval stages.Subject
Amino acid sequence
; DNA
; Embryos
; fish larvae
; gene expression
; Growth regulators; hormones
; larvae
; nucleotide sequence
; RNA
; cDNA
; cloning
; Growth hormone; mRNA; Milkfish
; Chanos chanos
; Early development
; mRNA expression














Taxonomic term
Collections
- AQD Journal Articles [1179]
Related items
Showing items related by title, author, creator and subject.
-
Cloning of mangrove red snapper (Lutjanus argentimaculatus) growth hormone cDNA and mRNA expression during early development
Samentar, Lorena P.; Ayson, Felix G.; de Jesus-Ayson, Evelyn Grace T.; Formacion, Minda J. (University of the Philippines Visayas, 2013)Growth hormone regulates growth and development in vertebrates. As a first step to understand the role of growth hormone in the regulation of growth and development of the mangrove red snapper Lutjanus argentimaculatus, ... -
mRNA expression patterns for GH, PRL, SL, IGF-I and IGF-II during altered feeding status in rabbitfish, Siganus guttatus.
Ayson, Felix G.; de Jesus-Ayson, Evelyn Grace T.; Takemura, Akihiro (Elsevier, 2007)Feeding time is a major synchronizer of many physiological rhythms in many organisms. Alteration in the nutritional status, specifically fasting, also affects the secretion rhythms of growth hormone (GH) and insulin-like ... -
Differential expression of insulin-like growth factor I and II mRNAs during embryogenesis and early larval development in rabbitfish, Siganus guttatus
Ayson, Felix G.; de Jesus, Evelyn Grace T.; Moriyama, Shunsuke; Hyodo, Susumu; Funkenstein, Bruria; Gertler, Arieh; Kawauchi, Hiroshi (Academic Press, 2002)In rodents, the expression of insulin-like growth factor II (IGF-II) is higher than that of insulin-like growth factor I (IGF-I) during fetal life while the reverse is true after birth. We wanted to examine whether this ...