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Characterization of betanodaviruses in the Philippines

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Associated URL
eafp.org
Date
2008
Author
de la Peña, Leobert D.
Mori, K.
Quinitio, Gerald F.
Chavez, Denny S.
Toledo, Joebert D.
Suarnaba, Vonnie S.
Maeno, Y.
Kiryu, I.
Nakai, T.
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Abstract
Viral nervous necrosis caused by betanodaviruses is one of the most devastating diseases in cultured marine finfish. In the Philippines, mass mortalities occurred in sea bass, Lates calcarifer larvae and grouper, Epinephelus coioides broodstock. The virus was isolated using SSN-1 fish cell line and confirmed by PCR. Cytopathic effect started to develop in the cell line 2 days post infection (p.i) with tissue filtrates until the cells completely disintegrated and detached from the flask at 5 days p.i. and the viral protein was detected by immunofluorescence. Sequence analysis revealed that VNN isolated from the brain of grouper broodstock and sea bass larvae were 98.6% similar. Sequence analysis between the Philippine isolates and red-spotted grouper nervous necrosis virus (RGNNV) genotype is 96.9% similar as compared to 72.0% and 64.0% similar with the barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV) genotypes, respectively. These results confirm that the Philippine isolates belong to RGNNV genotype.
URI
http://hdl.handle.net/10862/2076
Suggested Citation
de la Peña, L. D., Mori, K., Quinitio, G. F., Chavez, D. S., Toledo, J. D., Suarnaba, V. S., ... & Nakai, T. (2008). Characterization of betanodaviruses in the Philippines. Bulletin of the European Association of Fish Pathologists, 28(6), 230-237.
Subject
catadromous fishes ASFA; brain ASFA; immunofluorescence ASFA; genotypes ASFA; fish larvae ASFA; nucleotides ASFA; marine fish ASFA; nervous system ASFA; viral diseases ASFA; RNA ASFA; necrosis ASFA; polymerase chain reaction ASFA; mariculture ASFA; breeding stock ASFA; fish culture ASFA; Dicentrarchus labrax AGROVOC; Pleuronectiformes AGROVOC; Lates calcarifer AGROVOC; Epinephelus coioides AGROVOC; Philippines, Panay I., Iloilo
Taxonomic term
Lates calcarifer GBIF
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