Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis
MetadataShow full item record
Cited times in Scopus
γ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
Suggested CitationMarcial, H. S., Suga, K., Kinoshita, S., Kaneko, G., Hagiwara, A., & Watabe, S. (2014). Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis.
chemoreceptors ; antibodies ; marine invertebrates ; brain ; receptors ; proteins ; transcription ; hydrobiology ; evolution ; amino acids ; Reverse transcription; gamma -Aminobutyric acid receptors; GABARAP protein; Reviews; gamma -Aminobutyric acid A receptors; Membrane trafficking; Neonates; phagocytosis ; yeasts ; membranes ; acids ; Rotifera; Brachionus plicatilis; eggs
- AQD Journal Articles 
Showing items related by title, author, creator and subject.
Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile Seraspe, Ebonia B.; Gabotero, Shirleny; de la Peña, Milagros R.; Pahila, Ida G.; Amar, Edgar (Elsevier, 2014)Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and ...
Millamena, Oseni M. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2002)This section aims to teach the reader the ten essential amino acids required by fish and their chemical structures, distinguish between essential and non-essential amino acids; the fate of absorbed amino acids in fish; ...
Isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus) growth hormone Ayson, Felix G.; de Jesus, Evelyn Grace T.; Amemiya, Yutaka; Moriyama, Shunsuke; Hirano, Tetsuya; Kawauchi, Hiroshi (Elsevier, 2000)We report the isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus; Teleostei; Perciformes; Siganidae) growth hormone (GH). Rabbitfish GH was extracted from pituitary glands under alkaline ...