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Microalgal paste production of the diatom Chaetoceros calcitrans using electrolytic flocculation method at optimum culture conditions

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Date
2018
Author
de la Peña, Milagros R.
Franco, Annie Villa
Igcasan Jr., Hermoso P.
Arnaldo, Mary Dianne Grace N.
Piloton, Ramil M.
Garibay, Soledad S.
Balinas, Vicente T.
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Abstract
The optimum culture conditions of the local strain Chaetoceros calcitrans were determined to improve biomass and reduce cost of production. Under outdoor culture conditions, higher cell density was attained when the cultures were enriched with Tungkang Marine Research Laboratory (TMRL) medium composed of cheap technical grade reagents and cultured at 25 g L−1 salinity. The cultures were lighted with two 40 W cool-white GE fluorescent tubes (24–35 μmol photon m−2 s−1). Using semi-continuous culture system under established optimum culture conditions, C. calcitrans can be re-cultured thrice and concentrated at each culture cycle using electrolytic flocculation method to produce 4.6 kg m−3 of diatom paste. The viability of concentrated C. calcitrans after 3 months of storage was comparable to live diatom cells. Simple preservation technique by low-temperature storage is convenient for storing algal concentrates for use as starter cultures and for feeding invertebrates. The paste costs USD 8.24 kg−1 inclusive of the assets and flocculation materials for culturing and harvesting the diatom, respectively. This study established the suitable conditions for mass culture of C. calcitrans and produced concentrated diatoms in paste form that is readily available for aquaculture hatcheries at a lower cost.
URI
http://hdl.handle.net/10862/3291
Suggested Citation
de la Peña, M. R., Franco, A. V., Igcasan Jr., H. P., Arnaldo, M. D. G. N., Piloton, R. M., Garibay, S. S., & Balinas, V. T. (2018). Microalgal paste production of the diatom Chaetoceros calcitrans using electrolytic flocculation method at optimum culture conditions. Aquaculture International, 26(4), 1119-1134.
DOI
10.1007/s10499-018-0272-0
Subject
harvesting ASFA; strain ASFA; Reagents; cultures ASFA; marine sciences ASFA; storage ASFA; aquaculture ASFA; Algae ASFA; low temperature ASFA; flocculation ASFA; Invertebrates; Diatoms ASFA; mass culture ASFA; methodology ASFA; continuous culture ASFA; production costs ASFA; fish ASFA; Chaetoceros calcitrans; Microalgal paste; Electrolytic flocculation; Optimum culture conditions; hatcheries AGROVOC
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  • AQD Journal Articles [1179]

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