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dc.contributor.authorPakingking , Rolando, Jr.
dc.contributor.authorde Jesus-Ayson, Evelyn Grace
dc.contributor.authorDionela, Cleresa S.
dc.contributor.editorAya, Frolan A.
dc.contributor.editorde la Peña, Leobert D.
dc.contributor.editorSalayo, Nerissa D.
dc.contributor.editorTendencia, Eleonor A.
dc.date.accessioned2021-12-16T04:02:14Z
dc.date.available2021-12-16T04:02:14Z
dc.date.issued2021-12
dc.identifier.citationPakingking Jr., R. V., de Jesus-Ayson, E. G., & Dionela, C. (2021). Efficacy of the inactivated nervous necrosis virus vaccine against viral nervous necrosis in pond-reared orange-spotted grouper Epinephelus coioides. In F. A. Aya, L. D. de la Peña, N. D. Salayo, & E. A. Tendencia (Eds.), Proceedings of the International Workshop on the Promotion of Sustainable Aquaculture, Aquatic Animal Health, and Resource Enhancement in Southeast Asia (pp. 229–237). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9789719931102 (Print)
dc.identifier.isbn9789719931119 (PDF)
dc.identifier.urihttp://hdl.handle.net/10862/6276
dc.description.abstractThe field efficacy of the formalin-inactivated nervous necrosis virus (NNV) against viral nervous necrosis (VNN) in orange-spotted grouper (Epinephelus coioides) reared in floating net cages in earthen pond was investigated. Seroneutralization assay conducted on the sera of vaccinated fish exhibited the occurrence of neutralizing antibody titers from Day 30 (mean titer 1:1792±701) to Day 150 (1:704±351) with the highest titer observed at Day 60 (1:6656±3435) post-vaccination. Because mortality attributed to VNN was not encountered during the pond experiment, intramuscular challenges of vaccinated and unvaccinated (L-15 injected) fish with NNV (106.5 TCID50/fish) were conducted in indoor tanks at Day 30 (Mean body weight [MBW]: vaccinated [21±3.4 g]; unvaccinated [20.6±1 g) and Day 120 (MBW: vaccinated [178±27 g]; unvaccinated [176±19 g) postvaccination, respectively, to demonstrate the in vivo efficacy of the inactivated vaccine. Nil and 25 % mortality rate were obtained in vaccinated and control fish, respectively, challenged with NNV at Day 30 post-vaccination. On the contrary, nil mortality were obtained in both groups challenged with NNV at Day 120 post-vaccination. Although nil mortality was obtained in NNV-challenged unvaccinated fish, 30 % of the fish manifested dark coloration of the skin and abnormal swimming behavior that commenced and disappeared at Day 3 and Day 7 post-NNV challenge, respectively, suggesting an age/ weight-dependent resistance to the disease. Our current data illustrate that single vaccination with inactivated vaccine could mount the production of protective antibodies and concomitant conferment of protection against VNN in groupers especially during the early phase of grow-out culture in earthen ponds where they are highly susceptible to the disease.en
dc.description.sponsorshipThis study was funded by Government of Japan Trust Fund VI through the Regional Fish Disease Project (study code: FH01- F2015-T) and in part by SEAFDEC/ AQD. We express our heartfelt gratitude to Dr. Takuro Shibuno and Dr. Chihaya Nakayasu, former GOJ-TF managers, Dr. Koh Ichiro Mori, current GOJ-TF manager, and the Marine Fish Hatchery staff especially Mr. A. Gamuza.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 IGO*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/igo/*
dc.subjectInactivated vaccineen
dc.subjectviral nervous necrosisen
dc.subjectVNNen
dc.subjectEpinephelus coioidesen
dc.subjectgroupersen
dc.titleEfficacy of the inactivated nervous necrosis virus vaccine against viral nervous necrosis in pond-reared orange-spotted grouper Epinephelus coioidesen
dc.typeConference paperen
dc.citation.spage229
dc.citation.epage237
dc.subject.asfafishen
dc.subject.asfavirusesen
dc.subject.asfavaccinesen
dc.subject.asfavaccinationen
dc.subject.asfafish diseasesen
dc.subject.asfadiseasesen
dc.citation.conferenceTitleProceedings of the International Workshop on the Promotion of Sustainable Aquaculture, Aquatic Animal Health, and Resource Enhancement in Southeast Asiaen


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