Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status
MetadataShow full item record
Cited times in Scopus
Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the cDNAs for grouper GH and IGF-I and examined their mRNA expression during different nutritional status. Grouper GH cDNA is 936 base pairs (bp) long excluding the poly-A tail. It contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. It has an open reading frame of 612bp coding for a signal peptide of 17 amino acids (aa) and a mature hormone of 187aa residues. Based on the aa sequence of the mature hormone, grouper GH shows higher sequence identity (>76%) to GHs of perciforms than to GHs of cyprinids and salmonids (53-69%). Grouper preproIGF-I cDNA consisted of 558bp, which codes for 186aa. This is composed of 44aa for the signal peptide, 68aa for the mature peptide comprising B, C, A, and D domains, and 74aa for the E domain. Mature grouper IGF-I shows very high sequence identity to IGF-I of teleost fishes (84-97%) compared to advanced groups of vertebrates such as chicken, pig, and human (=<80%). Using DNA primers specific for grouper GH and IGF-I, the changes in mRNA levels of pituitary GH and hepatic IGF-I in response to starvation and refeeding were examined by a semi-quantitative RT-PCR. Significant elevation of GH mRNA level was observed after 2 weeks of food deprivation, and increased further after 3 and 4 weeks of starvation. GH mRNA level in fed-controls did not change significantly during the same period. Hepatic IGF-I mRNA level decreased significantly starting after 1 week of starvation until the 4th week. There was no significant change in IGF-I mRNA levels in fed-controls. One week of refeeding can restore the GH and IGF-I mRNA back to its normal levels. Deprivation of food for 1-4 weeks also resulted in cessation of growth and decrease in condition factor.
Suggested CitationPedroso, F. L., De Jesus-Ayson, E. G. T., Cortado, H. H., Hyodo, S., & Ayson, F. G. (2006). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status.
Hormones; Starvation; Nutrition; Endocrinology; Growth rate; Food availability; Marine fish; Condition factor; Growth hormone; Insulin-like growth factor I; Nutritional status; Gene expression; Dietary restrictions; Signal peptides; Liver; Polyadenylation; Amino acids; Base pairs; Pituitary; Polymerase chain reaction; Primers; Open reading frames; mRNA
- AQD Journal Articles 
Showing items related by title, author, creator and subject.
Emata, Arnil C. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1995)Most of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for ...
mRNA expression patterns for GH, PRL, SL, IGF-I and IGF-II during altered feeding status in rabbitfish, Siganus guttatus. Ayson, Felix G.; de Jesus-Ayson, Evelyn Grace T.; Takemura, Akihiro (Elsevier, 2007)Feeding time is a major synchronizer of many physiological rhythms in many organisms. Alteration in the nutritional status, specifically fasting, also affects the secretion rhythms of growth hormone (GH) and insulin-like ...
Garcia, LM (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996)Recent progress undertaken by SEAFDEC/AQD in the development of broodstock of a variety of cultured fish in the Philippines is reviewed. Spontaneous maturation and spawning has been achieved among captive breeders of ...