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dc.contributor.authorPedroso, Fiona L.
dc.contributor.authorDe Jesus-Ayson, Evelyn Grace T.
dc.contributor.authorCortado, Hanna H.
dc.contributor.authorHyodo, Susumu
dc.contributor.authorAyson, Felix G.
dc.date.accessioned2011-06-01T09:49:36Z
dc.date.available2011-06-01T09:49:36Z
dc.date.issued2006
dc.identifier.citationPedroso, F. L., de Jesus-Ayson, E. G. T., Cortado, H. H., Hyodo, S., & Ayson, F. G. (2006). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status. General and Comparative Endocrinology, 145(3), 237-246.en
dc.identifier.issn0016-6480
dc.identifier.urihttp://hdl.handle.net/10862/94
dc.description.abstractGrowth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the cDNAs for grouper GH and IGF-I and examined their mRNA expression during different nutritional status. Grouper GH cDNA is 936 base pairs (bp) long excluding the poly-A tail. It contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. It has an open reading frame of 612bp coding for a signal peptide of 17 amino acids (aa) and a mature hormone of 187aa residues. Based on the aa sequence of the mature hormone, grouper GH shows higher sequence identity (>76%) to GHs of perciforms than to GHs of cyprinids and salmonids (53-69%). Grouper preproIGF-I cDNA consisted of 558bp, which codes for 186aa. This is composed of 44aa for the signal peptide, 68aa for the mature peptide comprising B, C, A, and D domains, and 74aa for the E domain. Mature grouper IGF-I shows very high sequence identity to IGF-I of teleost fishes (84-97%) compared to advanced groups of vertebrates such as chicken, pig, and human (=<80%). Using DNA primers specific for grouper GH and IGF-I, the changes in mRNA levels of pituitary GH and hepatic IGF-I in response to starvation and refeeding were examined by a semi-quantitative RT-PCR. Significant elevation of GH mRNA level was observed after 2 weeks of food deprivation, and increased further after 3 and 4 weeks of starvation. GH mRNA level in fed-controls did not change significantly during the same period. Hepatic IGF-I mRNA level decreased significantly starting after 1 week of starvation until the 4th week. There was no significant change in IGF-I mRNA levels in fed-controls. One week of refeeding can restore the GH and IGF-I mRNA back to its normal levels. Deprivation of food for 1-4 weeks also resulted in cessation of growth and decrease in condition factor.en
dc.description.sponsorshipWe thank Jossette Bangcaya and Josephine Nocillado for providing us degenerate IGF-I reverse primer, Mr. Roman Sanares for the statistical analyses, Dr. Ebonia Seraspe for her valuable advice and help. This study was funded by SEAFDEC AQD to EGT de Jesus-Ayson (Nr-01-F2001T) and by a grant from USAID to FG Ayson (Grant No. TA-MOU-99-C19-001, Program in US-Israel Cooperative Development Research, Economic Growth).en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.ispartofGeneral and Comparative Endocrinology 145:237-246en
dc.subjectsomatotropinen
dc.subjectinsulin-like growth factoren
dc.subjectnutritional statusen
dc.subjectopen reading framesen
dc.titleChanges in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional statusen
dc.typeArticleen
dc.citation.volume145
dc.citation.issue3
dc.citation.spage237
dc.citation.epage246
dc.citation.journalTitleGeneral and Comparative Endocrinologyen
dc.subject.asfahormonesen
dc.subject.asfastarvationen
dc.subject.asfanutritionen
dc.subject.asfaendocrinologyen
dc.subject.asfagrowth rateen
dc.subject.asfafood availabilityen
dc.subject.asfamarine fishesen
dc.subject.asfacondition factoren
dc.subject.asfagene expressionen
dc.subject.asfaliveren
dc.subject.asfaamino acidsen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfaprimersen
dc.identifier.doi10.1016/j.ygcen.2005.09.001


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  • AQD Journal Articles [1213]
    These papers were contributed by AQD staff to various national and international journals

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