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A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation

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Date
1982
Author
Hara, Shiro
Canto Jr., Jose T.
Almendras, Jesus M. E.
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35
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Abstract
Various extenders, containing potassium chloride, sodium chloride, glucose, sodium citrate, Ringer's solution, cow serum and milkfish serum were used to preserve milkfish (Chanos chanos) sperm at near-zero temperatures (0-4°C) and in liquid nitrogen (?196°C). Milkfish serum was a superior extender in both cases. After 5 days, comparatively good motility (> 30%) and fertilizing capacity (6.7-18.9%) were observed in the near-zero liquid samples, while in other extenders, sperm ceased to show motility after 2 days. The fertilization success of 4-5 days cryopreserved sperm averaged 67.5% (n = 2) with milkfish serum, 60.5% (n = 2) with 400 mM glucose, 58.0% (n = 2) with 150 mM sodium chloride, 41.2% (n = 1) with Ringer's solution and 31.9% (n = 2) with cow serum.
URI
http://hdl.handle.net/10862/1041
Suggested Citation
Hara, S., Canto Jr., J. T., & Almendras, J. M. E. (1982). A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28(3-4), 339-346.
DOI
10.1016/0044-8486(82)90076-X
Subject
Chanos chanos AGROVOC; Sperm AGROVOC; Preservatives AGROVOC; Freezing storage; Aquaculture techniques AGROVOC; Milkfish AGROVOC; Fertilization AGROVOC
Taxonomic term
Chanos chanos GBIF
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