Isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus) growth hormone
ຄົ້ນຫາ / ເປີດ
Request this documentວັນທີ
2000ຜູ້ຂຽນ
Page views
138Metadata
ເບິ່ງບັນທຶກລາຍລະອຽດລາຍການCited times in Scopus
Share
ບົດຄັດຫຍໍ້
We report the isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus; Teleostei; Perciformes; Siganidae) growth hormone (GH). Rabbitfish GH was extracted from pituitary glands under alkaline conditions, fractionated by gel filtration chromatography on Sephadex G-100, and purified by high-performance liquid chromatography. The fractions containing GH were identified by immunoblotting with bonito GH antiserum. Under nonreducing conditions, the molecular weight of rabbitfish GH is about 19 kDa as estimated by SDS–PAGE. The purified hormone was potent in promoting growth in rabbitfish fry. Weekly intraperitoneal injections of the hormone significantly accelerated growth. This was evident 3 weeks after the start of the treatment, and its effect was still significant 2 weeks after the treatment was terminated. Rabbitfish GH cDNA was cloned to determine its nucleotide sequence. Excluding the poly (A) tail, rabbitfish GH cDNA is 860 base pairs (bp) long. It contained untranslated regions of 94 and 175 bp in the 5′ and 3′ ends, respectively. It has an open reading frame of 588 bp coding for a signal peptide of 18 amino acids and a mature protein of 178 amino acid residues. Rabbitfish GH has 4 cysteine residues. On the amino acid level, rabbitfish GH shows high identity (71–74%) with GHs of other perciforms, such as tuna, sea bass, yellow tail, bonito, and tilapia, and less (47–49%) identity with salmonid and carp GHs.
Suggested Citation
Ayson, F. G., de Jesus, E. G. T., Amemiya, Y., Moriyama, S., Hirano, T., & Kawauchi, H. (2000). Isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus) growth hormone. General and Comparative Endocrinology , 117(2), 251-259. https://doi.org/10.1006/gcen.1999.7409
ວິຊາ
Collections
- AQD Journal Articles [1215]
Related items
Showing items related by title, author, creator and subject.
-
Role of growth hormone in the adaptation to sea water of juveline brown trout, (Salmo trutta)
Almendras, J. M. E.; Punet, P. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996)The first part of the study investigates the ability of ovine growth hormone (oGH) to enhance the hypo-osmoregulatory and growth performance of juvenile brown trout after exposure to sea water (SW). Three groups of fish ... -
An evaluation of formulated diets for Nile tilapia fingerlings
Santiago, Corazon B.; Reyes, Ofelia S.; Aldaba, Mercedes B.; Laron, Manuel A. (Fisheries Research Society of the Philippines, 1986)Nine practical diets were formulated and prepared as dry pellet crumbles. These were fed to two batches of Nile tilapia fingerlings (0.753g initial mean body weigt for trial I, and 0.961g for trial II) at 10% of fish biomass ... -
Impact of AMPEP on the growth and occurrence of epiphytic Neosiphonia infestation on two varieties of commercially cultivated Kappaphycus alvarezii grown at different depths in the Philippines
Borlongan, Iris Ann G.; Tibubos, Keneth R.; Yunque, Dianne Aster T.; Hurtado, Anicia Q.; Critchley, Alan T. (Springer, 2011)Two varieties of the carrageenophyte Kappaphycus alvarezii (Tungawan, TUNG; and Giant tambalang, GTAM) from Zamboanga Sibugay, Philippines were used to test the efficacy of Acadian Marine Plant Extract Powder (AMPEP) as ...