SEAFDECINSTITUTIONAL REPOSITORY
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • English 
    • English
    • ไทย
    • 日本語
    • Bahasa Indonesia
    • Bahasa Melayu
    • Burmese
    • Filipino
    • Khmer
    • Lao
    • Tiếng Việt
  • Login
View Item 
  •   SEAFDEC Institutional Repository (SIR)
  • 03 SEAFDEC External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • AQD Journal Articles
  • View Item
  •   SEAFDEC Institutional Repository (SIR)
  • 03 SEAFDEC External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • AQD Journal Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA analysis

Thumbnail
View/Open
Request this document
Date
2004
Author
Romana-Eguia, Maria Rowena R.
Ikeda, Minoru
Basiao, Zubaida U.
Taniguchi, Nobuhiko
Page views
99
Metadata
Show full item record

Cited times in Scopus



Share 
 
Abstract
We analyzed microsatellite and mitochondrial DNA restriction fragment length polymorphism (mtDNA-RFLP) in two domesticated (NIFI and Israel) and four genetically improved (GIFT, GMT, FAC-selected and SEAFDEC-selected) Nile tilapia (Oreochromis niloticus) as well as five red hybrid tilapia (Oreochromis mossambicus × O. niloticus) stocks (BFS, FACred, NIFIred, HL, and PF) farmed in Asia. Microsatellite variation at five loci (UNH216, UNH172, UNH123, UNH147, UNH222) was more informative in characterizing stock differences than the mtDNA-RFLP markers that were based only on 14 restriction morphs. Contemporary microsatellite data showed that GIFT Nile tilapia had the highest mean expected heterozygosity (H>e=0.813), while GMT had the lowest (He=0.666). The unselected NIFI stock and SEAFDEC-selected were genetically similar, while GMT differed significantly from the other Nile tilapia stocks. Among the red tilapias, NIFIred had the highest He (0.715), while BFS had the lowest variability (He=0.567). The Taiwanese red tilapia HL and Thai NIFIred were genetically similar. Except for NIFI, most of the Nile and red tilapia stocks exhibited remarkably significant homozygote excess relative to Hardy–Weinberg Equilibrium (HWE), suggesting some degree of inbreeding. Asian Nile tilapias were more genetically diverse (pooled He=0.791; mtDNA nucleotide divergence value dA=0.009) than the red tilapias (pooled He=0.697; mean dA=0.004). This slight divergence between the Nile and red tilapias was also seen in the analysis of molecular variance (AMOVA; FCT=0.0018) and in genetic distance and nucleotide divergence dendrograms. However, the AMOVA revealed that the greater percentage of variation (99.33%) in the total genetic diversity of the surveyed stocks is principally due to differences at the individual level and not between nor within groups. The significance of these results is that they reflect and lead to new inferences regarding the selective breeding and culture methods used in managing these farmed stocks.
URI
http://hdl.handle.net/10862/2025
Suggested Citation
Romana-Eguia, M. R. R., Ikeda, M., Basiao, Z. U., & Taniguchi, N. (2004). Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA analysis. Aquaculture, 236(1-4), 131-150.
DOI
10.1016/j.aquaculture.2004.01.026
Subject
DNA ASFA; fish culture ASFA; Genetic diversity; hybrid culture ASFA; inbreeding ASFA; nucleotides ASFA; selective breeding ASFA; Oreochromis niloticus; Asia; Israel; Genetically improved stocks; Microsatellites; Mitochondrial DNA-RFLP; Tilapia
Collections
  • AQD Journal Articles [1175]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Application of DNA-based markers in stock enhancement programs 

    Romana-Eguia, Maria Rowena R. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2006)
    Aquaculture and fisheries management require tools for identifying individuals or groups of aquatic organisms for the purpose of monitoring performance (growth, survival and behavior) and stock structure. In aquaculture ...
  • Thumbnail

    Genetic diversity, population structure and demographic history of the tropical eel Anguilla bicolor pacifica in Southeast Asia using mitochondrial DNA control region sequences 

    Marini, Melfa; Pedrosa-Gerasmio, Ivane R.; Santos, Mudjekeewis; Shibuno, Takuro; Daryani, Ayu; Romana-Eguia, Maria Rowena R.; Wibowo, Arif (Elsevier, 2021-04)
    The tropical catadromous eel, Anguilla bicolor pacifica, an important fishery resource in Southeast Asia, is under threat due to overexploitation (especially of its glass eel phase) and the limited information on their ...
  • Thumbnail

    Genetic diversity and stock delineation of Philippine populations of the orange mud crab, Scylla olivacea 

    Paran, Faith Jessica M.; Ravago-Gotanco, Rachel June (Aquaculture Department, Southeast Asian Fisheries Development Center, 2017)
    The orange mud crab, Scylla olivacea, is regarded as an important fishery resource due to high demand and high market value. However, mud crab populations are threatened by over exploitation and habitat degradation, and ...

© SEAFDEC 2023
Contact Us
 

 

Browse

All of SIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics
Related Links
SEAFDEC/TD IRSEAFDEC/AQD IRSEAFDEC/MFRDMD IRSEAFDEC/IFRDMD IR

© SEAFDEC 2023
Contact Us