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dc.contributor.authorPakingking, Rolando V., Jr.
dc.contributor.authorSeron, R.
dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorMori, K.
dc.contributor.authorYamashita, H.
dc.contributor.authorNakai, T.
dc.date.accessioned2014-05-27T02:58:30Z
dc.date.available2014-05-27T02:58:30Z
dc.date.issued2009
dc.identifier.citationPakingking Jr., R., Seron, R., dela Peña, L., Mori, K., Yamashita, H., & Nakai, T. (2009). Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine. Journal of Fish Diseases, 32(5), 457-463.en
dc.identifier.issn0140-7775
dc.identifier.urihttp://hdl.handle.net/10862/2098
dc.description.abstractAsian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass.en
dc.description.sponsorshipWe would like to thank Dr. Gilda Lio-Po for giving valuable suggestions on this study. We also gratefully acknowledge Dr. Evelyn Grace de Jesus-Ayson and some staff of Finfish Hatchery and Biolab for their kind assistance during the experiment. This study was funded in part by the Government of Japan Trust Fund through the Regional Fish Disease Project (study code: 8001-T-FT-FH0507) and by a Grant-in-Aid for Scientific Research (No.18076) from the Ministry of Agriculture, Forestry and Fisheries of Japan.en
dc.language.isoenen
dc.publisherBlackwell Publishingen
dc.subjectDicentrarchus labraxen
dc.subjectLates calcariferen
dc.subjectJapanen
dc.subjectPhilippinesen
dc.titleImmune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccineen
dc.typeArticleen
dc.citation.volume32
dc.citation.issue5
dc.citation.spage457
dc.citation.epage463
dc.citation.journalTitleJournal of Fish Diseasesen
dc.subject.asfamarine fishen
dc.subject.asfaantibodiesen
dc.subject.asfafish diseasesen
dc.subject.asfaviral diseasesen
dc.subject.asfadisease controlen
dc.subject.asfavaccinesen
dc.subject.asfamortality causesen
dc.subject.asfamortalityen
dc.subject.asfanecrosisen
dc.subject.asfabrainen
dc.subject.asfakidneysen
dc.subject.asfavaccinationen
dc.identifier.essn1365-2761
dc.identifier.doi10.1111/j.1365-2761.2009.01040.x
dc.subject.scientificNameLates calcariferen


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  • AQD Journal Articles [1213]
    These papers were contributed by AQD staff to various national and international journals

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