Differential expression of insulin-like growth factor I and II mRNAs during embryogenesis and early larval development in rabbitfish, Siganus guttatus
![Thumbnail](https://repository.seafdec.org.ph/bitstream/10862/1918/3/1918-AysonFG2002.pdf.jpg)
ຄົ້ນຫາ / ເປີດ
Request this documentວັນທີ
2002ຜູ້ຂຽນ
Page views
160Metadata
ເບິ່ງບັນທຶກລາຍລະອຽດລາຍການCited times in Scopus
Share
ບົດຄັດຫຍໍ້
In rodents, the expression of insulin-like growth factor II (IGF-II) is higher than that of insulin-like growth factor I (IGF-I) during fetal life while the reverse is true after birth. We wanted to examine whether this is also true in fish and whether IGF-I and IGF-II are differentially regulated during different stages of embryogenesis and early larval development in rabbitfish. We first cloned the cDNAs of rabbitfish IGF-I and IGF-II from the liver. Rabbitfish IGF-I has an open reading frame of 558 bp that codes for a signal peptide of 44 amino acids (aa), a mature protein of 68 aa, and a single form of E domain of 74 aa. Rabbitfish IGF-II, on the other hand, has an open reading frame of 645 bp that codes for a signal peptide of 47 aa, a mature protein of 70 aa, and an E domain of 98 aa. On the amino acid level, rabbitfish IGF-I shares 68% similarity with IGF-II. We then examined the relative expression of the two IGFs in unfertilized eggs, during different stages of embryogenesis, and in early larval stages of rabbitfish by a semiquantitative reverse transcription-polymerase chain reaction. Primers that amplify the mature peptide region of both IGFs were used and PCR for both peptides was done simultaneously, with identical PCR conditions for both. The identity of the PCR products was confirmed by direct sequencing. Contrary to published reports for seabream and rainbow trout, IGF-I mRNA was not detected in rabbitfish unfertilized eggs; it was first expressed in larvae soon after hatching. IGF-II mRNA, however, was expressed in unfertilized eggs, albeit weakly, and was already strongly expressed during the cleavage stage. mRNAs for both peptides were strongly expressed in the larvae, although IGF-II mRNA expression was higher than IGF-I expression.
Suggested Citation
Ayson, F. G., de Jesus, E. G. T., Moriyama, S., Hyodo, S., Funkenstein, B., Gertler, A., & Kawauchi, H. (2002). Differential expression of insulin-like growth factor I and II mRNAs during embryogenesis and early larval development in rabbitfish, Siganus guttatus. General and Comparative Endocrinology , 126(2), 165-174. https://doi.org/10.1006/gcen.2002.7788
ວິຊາ
Collections
- AQD Journal Articles [1222]
Related items
Showing items related by title, author, creator and subject.
-
Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status
Pedroso, Fiona L.; De Jesus-Ayson, Evelyn Grace T.; Cortado, Hanna H.; Hyodo, Susumu; Ayson, Felix G. (Elsevier, 2006)Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the ... -
Determination of physical properties of meat: Measurement of free and expressible drips
Ng, Cher Siang (Marine Fisheries Research Department, Southeast Asian Fisheries Development Center, 1987)Presented in the paper is the standard procedure in the measurement of free and expressible drips. Specifically, the required apparatus, and the analytical procedures and calculations for free and expressible drips are presented. -
Dietary P regulates phosphate transporter expression, phosphatase activity, and effluent P partitioning in trout culture
Coloso, R. M.; King, K.; Fletcher, J. W.; Weis, P.; Werner, A.; Ferraris, R. P. (Springer Verlag, 2003)Phosphate utilization by fish is an important issue because of its critical roles in fish growth and aquatic environmental pollution. High dietary phosphorus (P) levels typically decrease the efficiency of P utilization, ...