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  •   SEAFDEC Institutional Repository (SIR)
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Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay

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Associated URL
www.hvm.bioflux.com.ro
Date
2012
Author
Caipang, Christopher Marlowe A.
Pakingking , Rolando, Jr.
Apines-Amar, Mary Jane S.
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38
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Abstract
The aim of this study was to standardize a loop-mediated isothermal amplification (LAMP) assay for the detection of Vibrio harveyi , the causative agent of vibriosis in Asian seabass, Lates calcarifer. The dnaJ gene of the bacterial pathogen was used as the target gene for the LAMP assay. It was optimized at an incubation time of 1 h at 63°C. The assay was highly specific for V. harveyi and did not cross-react with other bacterial pathogens offish. However, the assay was able to detect V. harveyi that was isolated from infected shrimps. The limit of detection of the LAMP assay was 40 pg of DNA mL-1 or 40 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting the bacterial pathogen from infected samples. The LAMP products can be quantified spectrophotometrically using hydroxynaphthol blue (HNB) dye and showed positive correlation with the amount of the pathogen. These results demonstrated that LAMP is a simple and sensitive detection technique that has potential application for routine diagnosis of vibrosis caused by V. harveyi in Asian seabass and other aquatic species.
URI
http://hdl.handle.net/10862/2172
Suggested Citation
Caipang, C. M. A., Pakingking , R. Jr., & Apines-Amar, M. J. S. (2012). Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay. Human & Veterinary Medicine, 4(2), 52-57.
Subject
vibriosis ASFA; aquatic animals ASFA; aquatic organisms ASFA; bio-assays ASFA; polymerase chain reaction ASFA; screening ASFA; detection ASFA; LAMP AGROVOC; sea bass AGROVOC; Lates calcarifer AGROVOC; diagnostic techniques AGROVOC; diagnosis AGROVOC
Taxonomic term
Vibrio harveyi GBIF; Lates calcarifer GBIF
Collections
  • AQD Journal Articles [1158]

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