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Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos

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Date
2015
Author
Santos, Brian S.
Romana-Eguia, Maria Rowena R.
Basiao, Zubaida U.
Ikeda, Minoru
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Abstract
The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
URI
http://hdl.handle.net/10862/2266
Suggested Citation
Santos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos. Conservation Genetics Resources, 7(2), 451-453. https://doi.org/10.1007/s12686-014-0393-3 
DOI
10.1007/s12686-014-0393-3
Subject
Microsatellite markers; Population genetic structure; milkfish; Chanos chanos
Taxonomic term
Chanos chanos
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  • AQD Journal Articles [1249]

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