Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, Schizochytrium sp. LEY7 using low-cost substrates at optimum culture conditions
Share
抄録
This study was conducted to investigate low-cost substrate alternative and the optimum culture conditions in mass producing the local marine thraustochytrid, Schizochytrium sp. LEY7 isolated from the mangrove leaves of Baybay, Southern Leyte Philippines. Results showed that Schizochytrium sp. LEY7 is able to utilize commercial grade glucose and yeast extract from NaCl-treated baker's yeast as source of carbon and micronutrients respectively. Cost of mass producing the thraustochytrid isolate using the alternative production substrates was substantially reduced. Incubation temperature and salinity levels were the two growth factors significantly affecting the biomass production of the isolate. The short duration of lag phase shown by the isolate suggests a growth advantage in that cells are readily able to adapt to their new environment. Total lipids averaged to 19.4%. Principal fatty acids were palmitic acid (C16:0) with 33.52% and docosahexanoic acid (DHA, C22:6n-3) with 39.92% proportion to total fatty acid. Eicosapentaenoic (C20:5n-3, 1.01%) and arachidonic (C20:4n-3, 0.90%) are present but in relatively lower amount. Our findings showed the potential of low-cost substrate in mass producing the local thraustochytrid isolate, Schizochytrium sp. LEY7 as lipid and polyunsaturated fatty acid source in aquaculture. Biomass production was enhanced by optimizing the culture conditions.
Suggested Citation
Ludevese-Pascual, G., de la Peña, M. R., & Tornalejo, J. (2016). Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, Schizochytrium sp. LEY7 using low-cost substrates at optimum culture conditions. Aquaculture Research , 47(1), 318-328. https://doi.org/10.1111/are.12494
主題
Collections
- AQD Journal Articles [1223]
Related items
Showing items related by title, author, creator and subject.
-
Preliminary findings on supplementation of snapper fry, Lutjanus sp. with DHA-rich thraustochytrid strain, Schizochytrium sp. LEY7 showed increased growth, survival and robustness
Ludevese-Pascual, Gladys (Southern Leyte State University, 2018)In this study, a preliminary experiment was conducted to investigate the beneficial effects of an indigenous DHA-rich thraustochytrid strain, Schizochytrium sp. LEY7 as supplement for snapper fry Lutjanus sp. using a ... -
Adsorption and biomass concentration of thraustochytrid Schizochytrium aggregatum (Goldstein and Belsky) in Bunker C Oil
Sarinas, Brian Gil S.; Gellada, Lorna D.; Torrigue, Ma. Lona T.; Sibonga, Dolores N.; Torrato, Edmar S.; Malagad, John G.; Feril, Joseph G.; Bondoc, Lyle Arianne J.; Roncal, Juan Clemente A.; Tornalejo, Jilla A. (School of Environmental Science and Management, University of the Philippines Los Baños, 2014)Diverse array of microorganisms such as bacteria, fungi and protists are involved during oil spill. Each microorganism has its own specific function whether it has to degrade or adsorb hydrocarbons. One important microorganism ... -
Growth and fatty acid production of thraustochytrids from Panay mangroves, Philippines
Leaño, Eduardo M.; Gapasin, Rolando S. J.; Polohan, Bernice; Vrijmoed, Lilian L.P. (Mushroom Research Foundation, 2003)Optimization of culture conditions with regard to the combined effects of salinity and temperature on biomass and fatty acid production of four thraustochytrid isolates were undertaken. Two strains of Schizochytrium mangrovei ...