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GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis

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រកមើល / បើកOpen
កាលបរិច្ឆេទ
2000
អ្នកនិពន្ធ
Gallardo, Wenresti G.
Hagiwara, Atsushi
Hara, Kenji
Soyano, Kiyoshi
Snell, Terry W.
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556
ទិន្នន័យមេតា
មើលកំណត់ត្រាលម្អិតនៃធាតុ

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អរូបី
γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids.
Keywords
Amino acids Brachionus Dot blot immunoassay 5-HT GABA HPLC Neurotransmitters Rotifera Serotonin
URI
http://hdl.handle.net/10862/1870
Suggested Citation
Gallardo, W. G., Hagiwara, A., Hara, K., Soyano, K., & Snell, T. W. (2000). GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 127(3), 301-307. https://doi.org/10.1016/S1095-6433(00)00266-X 
DOI
10.1016/S1095-6433(00)00266-X
ប្រធានបទ
amino acids; biochemical composition; reproductive cycle; HPLC; neurotransmitters; Brachionus plicatilis; Brachionus rotundiformis; Dot blot immunoassay; 5-HT; gamma-aminobutyric acid; Rotifera; serotonin
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