Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines
![Thumbnail](https://repository.seafdec.org.ph/bitstream/10862/2016/3/2016-MaenoY2004.pdf.jpg)
Associated URL
www.jircas.affrc.go.jpDate
2004Page views
229Metadata
Show full item recordCited times in Scopus
Share
Abstract
Viral nervous necrosis (VNN), also known as viral encephalopathy and retinopathy (VER), is an emerging disease affecting larvae and juveniles of many farmed marine fish species in Asia, Australia, Europe and North America. Mass mortality occurred in 14-day old larval sea bass Lates calcarifer at a hatchery in the Philippines associated with clinical signs such as abnormal swimming behavior and pale-gray discoloration of the body. Histological investigations in moribund fish revealed marked vacuolation in the retina and brain. Cytopathic effects (CPE) were observed in SSN-1 cells inoculated with the tissue filtrate of affected sea bass. A piscine nodavirus, the causative agent of VNN, was detected in the affected tissues and SSN-1 cells inoculated with the tissue filtrate of affected fish by RT-PCR. Electron microscopy revealed non-enveloped viral particles, 22-28 nm in diameter, in the cytoplasm of the brain and retina of affected fish and in the cytoplasm of VNN-infected SSN-1 cells after CPE appeared. These results indicate that mass mortality of sea bass larvae in the Philippines was caused by a piscine nodavirus.
Suggested Citation
Maeno, Y., de la Peña, L. D., & Cruz-Lacierda, E. R. (2004). Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines. Japan Agricultural Research Quarterly , 38(1), 69-73. https://doi.org/10.6090/jarq.38.69
Subject
fish kill
; hatching
; infectious diseases
; necrosis
; viruses
; Lates calcarifer
; Philippines
; VNN; VER
; Piscine nodavirus
![ASFA ASFA](/themes/Mirage2/images/AGROVOC-logo.gif)
![ASFA ASFA](/themes/Mirage2/images/AGROVOC-logo.gif)
![ASFA ASFA](/themes/Mirage2/images/AGROVOC-logo.gif)
![ASFA ASFA](/themes/Mirage2/images/AGROVOC-logo.gif)
![ASFA ASFA](/themes/Mirage2/images/AGROVOC-logo.gif)
![AGROVOC AGROVOC](/themes/Mirage2/images/AGROVOC-logo.gif)
![AGROVOC AGROVOC](/themes/Mirage2/images/AGROVOC-logo.gif)
![AGROVOC AGROVOC](/themes/Mirage2/images/AGROVOC-logo.gif)
Taxonomic term
Collections
- AQD Journal Articles [1222]
Related items
Showing items related by title, author, creator and subject.
-
Seabass grow-out and marketing: lessons from Australia, Malaysia, and Thailand
Buendia, Romeo (Aquaculture Department, Southeast Asian Fisheries Development Center, 1997) -
Optimized feeding strategies in the larviculture of the Asian seabass Lates calcarifer.
Dhert, Philippe.; Duray, Marietta; Lavens, Patrick.; Sorgeloos, Patrick. (Asian Fisheries Society, 1990)This paper reports on the progress made at the Tigbauan hatchery of SEAFDEC in the Philippines, with the larviculture of the Asian seabass Lates calcarifer when using w3-HUFA enriched Brachionus and Artemia . In view of ... -
Illuminated-cage nursery of the Asian sea bass, Lates calcarifer Bloch, (Centropomidae): effects of initial body size and stocking density
Fermin, Armando C. (Asian Fisheries Society; World Aquaculture Society - Southeast Asian Chapter, 2000)This study was conducted to determine the appropriate initial body size and the corresponding stocking density of sea bass, Lates calcarifer, during nursery rearing in illuminated cages. Hatchery-produced sea bass fry of ...