Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).

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Abstract
A convenient and rapid biopsy method for taking ovarian samples from mature sea bass (Lates calcarifer Bloch) is described. Intra-ovarian oocytes siphoned with polyethylene tubing from any region of the ovary provide a sample representative of the maturational stage of sea bass. The osmolality of a 5% phosphate buffered formalin solution is similar to that of sea bass plasma. The follicular diameter of cannulated sea bass oocytes can be measured within an hour after fixation in 5% phosphate buffered formalin without significantly deviating from the diameter of fresh oocytes.
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Garcia, L. M. (1989). Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch). Aquaculture , 77(1), 97-102. https://doi.org/10.1016/0044-8486(89)90025-2
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